지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 52 - 65 (14page)
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초록· 키워드
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Objective: Toad venom, called Chan-Su, is a traditional Oriental medicine secreted from the auricular and the skin glands of the Bufo bufo gargarizanz Cantor or B. melanosticus Schneider and has been widely used in China, Korea and other parts of Asia for the treatment of pain, heart conditions, and cancer. We examined the concentrations of the main chemical constituents within a commercially-available toad venom product and compared the levels for different extraction methods.
Methods: Toad venom was extracted using either cold or hot water, ethanol (EtOH), methanol (MeOH), or ethyl acetate (EtOAc), was fractionated using precipitation or reflux, and was then analyzed using thin layer chromatography (TLC), high-performance liquid chromatography (HTLC), and liquid chromatography - mass spectrometry (LC-MS). Individual components were identified by comparisons of the retention times, the ultraviolet spectra, and mass spectras and differences in chemical constituents for different solvents and extraction methods are presented.
Results: Components with authentic standards, including serotonin and bufodienolides (cinobufagen, bufalin, cinobufalin, and resibufogenin), were detected. The water extract of toad venom contained the greatest amount of serotonin (75.7 ± 0.1 mg/g), but very small amounts of bufodienolides (3.8 ± 0.0 mg/g). In contrast, the use of MeOH or EtOH extraction solutions resulted in 5-26 times higher concentrations of bufodienolides, with only trace amounts of serotonin. The relative and the absolute concentrations of the component also varied based on the extraction method; i.e., EtOH extracts yielded the greatest total amounts of bufodienolides, and EtOAc precipitation had the lowest amounts of bufodienolides.
Conclusions: Toad venom consists of serotonin and several bufodienolides, and the choice of solvent to extract chemical the constituents is important as a way to enrich the purported active components for treating different conditions.
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참고문헌 (21)
참고문헌 신청
Akizawa T. / 1994 / Structures of novel bufadienolides in the eggs of a toad, Bufo marinus / Chem Pharm Bull 42 (3) : 754 ~ 756
Barry TL. / 1996 / GC/MS Comparison of the west Indian Aphrodisiac"Love Stone"to the Chinese Medication"Chan Su" : Bufotenine and related Bufodienolides / J Forensic Sci 41
Chan WY. / 1995 / Examination for toxicity of a Chinese drug, the total glandular secretion product Chan Su in pregnant mice and embryos / Biol Neonate 67 (5) : 376 ~ 380
Chen KK. / 1967 / Pharmacology and toxicology of toad venom / J Pharm Sci 56 (12) : 1535 ~ 1541
Das M. / 1998 / Immunomodulatory and antineoplastic activity of common Indian toad(Bufo melanostictus, Schneider)skin extract / Indian J Pharmacol 30 (5) : 311 ~ 317
Barry TL. / 1996 / GC/MS Comparison of the west Indian Aphrodisiac"Love Stone"to the Chinese Medication"Chan Su" : Bufotenine and related Bufodienolides / J Forensic Sci 41
Chan WY. / 1995 / Examination for toxicity of a Chinese drug, the total glandular secretion product Chan Su in pregnant mice and embryos / Biol Neonate 67 (5) : 376 ~ 380
Chen KK. / 1967 / Pharmacology and toxicology of toad venom / J Pharm Sci 56 (12) : 1535 ~ 1541
Das M. / 1998 / Immunomodulatory and antineoplastic activity of common Indian toad(Bufo melanostictus, Schneider)skin extract / Indian J Pharmacol 30 (5) : 311 ~ 317
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