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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 31 - 38 (8page)
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초록· 키워드
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Sophorae Radix (SR) plays a role in a number of physiologic and pharmacologic functions in many organs.
Objective: The aim of this study was to clarify the potential role for transient receptor potential melastatin 7 (TRPM7)channels in SR-inhibited growth and survival of AGS and MCF-7 cells, the most common human gastric and breast adenocarcinoma cell lines.
Methods: The AGS and the MCF-7 cells were treated with varying concentrations of SR. Analyses of the caspase-3 and -9 activity, the mitochondrial depolarization and the poly (ADPribose) polymerase (PARP) cleavage were conducted to determine if AGS and MCF-7 cell death occured by apoptosis.
TRPM7 channel blockers (Gd3+ or 2-APB) and small interfering RNA (siRNA) were used in this study to confirm the role of TRPM7 channels. Furthermore, TRPM7 channels were overexpressed in human embryonic kidney (HEK) 293 cells to identify the role of TRPM7 channels in AGS and MCF-7 cell growth and survival.
Results: The addition of SR to a culture medium inhibited AGS and MCF-7 cell growth and survival. Experimental results showed that the caspase-3 and -9 activity, the mitochondrial depolarization, and the degree of PARP cleavage was increased. TRPM7 channel blockade, either by Gd3+ or 2-APB or by suppressing TRPM7 expression with small interfering RNA, blocked the SR-induced inhibition of cell growth and survival. Furthermore, TRPM7 channel overexpression in HEK 293 cells exacerbated SR-induced cell death.
Conclusions: These findings indicate that SR inhibits the growth and survival of gastric and breast cancer cells due to a blockade of the TRPM7 channel activity. Therefore, TRPM7channels may play an important role in the survival of patients with gastric and breast cancer.
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참고문헌 (26)
참고문헌 신청
Abdul M. / 2002 / Voltage-gated potassium ion channels in colon cancer / Oncol Rep 9 (5) : 961 ~ 964
Abdul M. / 2002 / Voltage-gated sodium ion channels in prostate cancer : expre
Clapham DE. / 2003 / TRP channels as cellular sensors / Nature 426 (6966) : 517 ~ 524
De Naeyer A. / 2004 / Estrogenic and anticarcinogenic properties of kurarinone, a lavandulyl flavanone from the roots of Sophora flavescens / J Nat Prod 67 (11) : 1829 ~ 183
Ding P. / 2004 / Cytotoxic isoprenylated flavonoids from the roots of Sophora flavescens / Helv Chim Acta 87 (10) : 2574 ~ 2580
Abdul M. / 2002 / Voltage-gated sodium ion channels in prostate cancer : expre
Clapham DE. / 2003 / TRP channels as cellular sensors / Nature 426 (6966) : 517 ~ 524
De Naeyer A. / 2004 / Estrogenic and anticarcinogenic properties of kurarinone, a lavandulyl flavanone from the roots of Sophora flavescens / J Nat Prod 67 (11) : 1829 ~ 183
Ding P. / 2004 / Cytotoxic isoprenylated flavonoids from the roots of Sophora flavescens / Helv Chim Acta 87 (10) : 2574 ~ 2580
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