지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 19 - 30 (12page)
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Objectives: Many efforts have shown multi-oncologic roles of galectin-3 for cell proliferation, angiogenesis, and apoptosis.
However, the mechanisms by which galectin-3 is involved in cell proliferation are not yet fully understood, especially in human colon cancer cells.
Methods: To cluster genes showing positively or negatively correlated expression with galectin-3, we employed human colon cancer cell lines, SNU-61, SNU-81, SNU-769B, SNU-C4and SNU-C5 in high-throughput gene expression profiling.
Gene and protein expression levels were determined by using real-time quantitative polymerase chain reaction (PCR) and western blot analysis, respectively. The proliferation rate of human colon cancer cells was measured by using a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT)assay.
Results: Expression of γ-aminobutyric acid B receptor 1(GABABR1) showed a positive correlation with galectin-3 at both the transcriptional and the translational levels. Downregulation of galectin-3 decreased not only GABABR1expression but also the proliferation rate of human colon cancer cells. However, Korean herbal extract, HangAmDan-B (HAD-B), decreased expression of GABABR1 without any expressional change of galectin-3, and offset γ-aminobutyric acid (GABA)-enhanced human colon cancer cell proliferation.
Conclusions: Our present study confirmed that GABABR1expression was regulated by galectin-3. HAD-B induced galectin-3-independent down-regulation of GABABR1, which resulted in a decreased proliferation of human colon cancer cells. The therapeutic effect of HAD-B for the treatment of human colon cancer needs to be further validated.
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참고문헌 (32)
참고문헌 신청
Li J. / 2009 / Inhibition of autophagy by 3-MA enhances the effect of 5-FUinduced apoptosis in colon cancer cells / Ann Surg Oncol 16(3):761~771
Padgett CL. / 2010 / GABAB receptor coupling to Gproteins and ion channels / Adv Pharmacol 58:123~147
Kaupmann K. / 1997 / Expression cloning of GABA(B)receptors uncovers similarity to metabotropic glutamate receptors / Nature 386(6622):239~246
Kaupmann K. / 1998 / GABA(B)-receptor subtypes assemble into functional heteromeric complexes / Nature 396(6712):683~687
Schuller HM. / 2008 / Gamma-aminobutyric acid, a potential tumor suppressor for small airway-derived lung adenocarcinoma / Carcinogenesis 29(10):1979~1985
Padgett CL. / 2010 / GABAB receptor coupling to Gproteins and ion channels / Adv Pharmacol 58:123~147
Kaupmann K. / 1997 / Expression cloning of GABA(B)receptors uncovers similarity to metabotropic glutamate receptors / Nature 386(6622):239~246
Kaupmann K. / 1998 / GABA(B)-receptor subtypes assemble into functional heteromeric complexes / Nature 396(6712):683~687
Schuller HM. / 2008 / Gamma-aminobutyric acid, a potential tumor suppressor for small airway-derived lung adenocarcinoma / Carcinogenesis 29(10):1979~1985
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