지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 13 - 18 (6page)
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Objectives: The purpose of this study is to examine whether Hominis Placental pharmacopuncture solution (HPPS) combined with zinc-oxide nanoparticles (ZnO NP) activates RAW 264.7 cells.
Methods: We soaked ZnO nanoparticles in the Hominis Placenta pharmacopuncture solution, thereby making a combined form (ZnO NP HPPS). The effect of ZnO NP HPPS on the intracellular reactive oxygen species (ROS) production was measured by 2', 7'-dichlorofluorescin diacetate (DCFH-DA) assay.
The effect of ZnO NP HPPS on NF-κB was measured by using a luciferase assay. The effect of ZnO NP HPPS on the cytokine expression was assessed by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR). The cellular uptake of ZnO NP HPPS was measured by using a flow cytometric analysis, and cellular structural alterations were analyzed by using transmission electron microscopy (TEM).
Results: Neither the HPPS nor the ZnO NPs induced intracellular ROS production in RAW 264.7 cells. Neither of the materials activated NF-κB or it’s dependent genes, such as TNF-α,IL-1, and MCP-1. However, ZnO NP HPPS, the combined form of ZnO NPs and HPPS, did induce the intracellular ROS production,as well as prominently activating NF-κB and it’s dependent genes. Also, compared to ZnO NPs, it effectively increased the uptake by RAW 264.7 cells. In addition, cellular structural alterations were observed in groups treated with ZnO NP HPPS.
Conclusions: Neither ZnO NP nor HPPS activated RAW 264.7cells, which is likely due to a low cellular uptake. The ZnO NP HPPS, however, significantly activated NF-κB and up-regulated its dependent genes such as TNF-α, IL-1, and MCP-1.
ZnO NP HPPS was also more easily taken into the RAW 264.7cells than either ZnO NP or HPPS.
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참고문헌 (27)
참고문헌 신청
Gilmore TD. / 2006 / Introduction to NF-kappaB : players, pathways, perspectives / Oncogene 25(51):6680~6684
Brasier AR. / 2006 / The NF-kappaB regulatory network / Cardiovasc Toxicol 6(2):111~130
Tsou TC. / 2010 / Zinc oxide particles induce inflammatory responses in vascular endothelial cells via NF-κB signaling / J Hazard Mater. 183(1-3):182~188
Leto TL. / 2006 / Role of Nox family NADPH oxidases in host defense / Antioxid Redox Signal 8(9-10):1549~1561
Brigger I. / 2002 / Nanoparticles in cancer therapy and diagnosis / Adv Drug Delivery Rev 54 (5) : 631 ~ 651
Brasier AR. / 2006 / The NF-kappaB regulatory network / Cardiovasc Toxicol 6(2):111~130
Tsou TC. / 2010 / Zinc oxide particles induce inflammatory responses in vascular endothelial cells via NF-κB signaling / J Hazard Mater. 183(1-3):182~188
Leto TL. / 2006 / Role of Nox family NADPH oxidases in host defense / Antioxid Redox Signal 8(9-10):1549~1561
Brigger I. / 2002 / Nanoparticles in cancer therapy and diagnosis / Adv Drug Delivery Rev 54 (5) : 631 ~ 651
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