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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 32 - 41 (10page)
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Objectives: Condurango (Gonolobus condurango) extract is used by complementary and alternative medicine (CAM) practitioners as a traditional medicine, including homeopathy, mainly for the treatment of syphilis. Condurango bark extract is also known to reduce tumor volume, but the underlying molecular mechanisms still remain unclear.
Methods: Using a cervical cancer cell line (HeLa) as our model, the molecular events behind condurango extract’s (CE’s) anticancer effect were investigated by using flow cytometry, immunoblotting and reverse transcriptase-polymerase chain reaction (RT-PCR). Other included cell types were prostate cancer cells (PC3), transformed liver cells (WRL-68), and peripheral blood mononuclear cells (PBMCs).
Results: Condurango extract (CE) was found to be cytotoxic against target cells, and this was significantly deactivated in the presence of N-acetyl cysteine (NAC), a scavenger of reactive oxygen species (ROS), suggesting that its action could be mediated through ROS generation. CE caused an increase in the HeLa cell population containing deoxyribonucleic acid (DNA) damage at the G zero/Growth 1 (G0/G1) stage. Further, CE increased the tumor necrosis factor alpha (TNF-α) and the fas receptor (FasR) levels both at the ribonucleic acid (RNA) and the protein levels, indicating that CE might have a cytotoxic mechanism of action. CE also triggered a sharp decrease in the expression of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB ) both at the RNA and the protein levels, a possible route to attenuation of B-cell lymphoma 2 (Bcl-2), and caused an opening of the mitochondrial membrane’s permeability transition (MPT) pores, thus enhancing caspase activities.
Conclusion: Overall, our results suggest possible pathways for CE mediated cytotoxicity in model cancer cells.
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참고문헌 (25)
참고문헌 신청
[학술지(정기간
Bishayee K / 2013 / Condurango-glycoside-A fraction of Gonolobus condurango induces DNA damage associated senescence and apoptosis via ROS-dependent p53 signalling pathway in
Chen J / 2008 / Identification of polyoxypregnane glycosides from the stems of Marsdenia tenacissima by high-performance liquid chromatography/tandem mass spectrometry / Tal
Fiandalo MV / 2012 / Caspase control: protagonists of cancer cell apoptosis / Exp Oncol 34 (3) : 165 ~ 175
Fulda S / 2010 / Cellular stress responses: cell survival and cell death / Int J Cell Biol 2010
Bishayee K / 2013 / Condurango-glycoside-A fraction of Gonolobus condurango induces DNA damage associated senescence and apoptosis via ROS-dependent p53 signalling pathway in
Chen J / 2008 / Identification of polyoxypregnane glycosides from the stems of Marsdenia tenacissima by high-performance liquid chromatography/tandem mass spectrometry / Tal
Fiandalo MV / 2012 / Caspase control: protagonists of cancer cell apoptosis / Exp Oncol 34 (3) : 165 ~ 175
Fulda S / 2010 / Cellular stress responses: cell survival and cell death / Int J Cell Biol 2010
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