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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 36 - 49 (14page)
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초록· 키워드
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Objectives: Quercetin and curcuminoids are importantbioactive compounds found in many herbs. Previouslyreported high performance liquid chromatographyultraviolet (HPLC-UV) methods for the detection ofquercetin and curcuminoids have several disadvantages,including unsatisfactory separation times and lack ofvalidation according the standard guidelines of the InternationalConference on Harmonisation of TechnicalRequirements for Registration of Pharmaceuticals forHuman Use.
Methods: A rapid, specific, reversed phase, HPLC-UVmethod with an isocratic elution of acetonitrile and 2%v/v acetic acid (40% : 60% v/v) (pH 2.6) at a flow rate of1.3 mL/minutes, a column temperature of 35°C, and ultraviolet(UV) detection at 370 nm was developed. Themethod was validated and applied to the quantificationof different types of market available Chinese medicineextracts, pills and tablets.
Results: The method allowed simultaneous determinationof quercetin, bisdemethoxycurcumin, demethoxycurcuminand curcumin in the concentration ranges of 0.00488 ─ 200 μg/mL, 0.625 ─ 320 μg/mL, 0.07813─ 320 μg/mL and 0.03906 ─ 320 μg/mL, respectively.
The limits of detection and quantification, respectively,were 0.00488 and 0.03906 μg/mL for quercetin,0.62500 and 2.50000 μg/mL for bisdemethoxycurcumin,0.07813 and 0.31250 μg/mL for demethoxycurcumin,and 0.03906 and 0.07813 μg/mL for curcumin. Thepercent relative intra day standard deviation (% RSD)values were 0.432 ─ 0.806 μg/mL, 0.576 ─ 0.723 μg/mL, 0.635 ─ 0.752 μg/mL and 0.655 ─ 0.732 μg/mLfor quercetin, bisdemethoxycurcumin, demethoxycurcuminand curcumin, respectively, and those forintra day precision were 0.323 ─ 0.968 μg/mL, 0.805 ─0.854 μg/mL, 0.078 ─ 0.844 μg/mL and 0.275 ─ 0.829μg/mL, respectively. The intra day accuracies were99.589% ─ 100.821%, 98.588% ─ 101.084%, 9.289% ─100.88%, and 98.292% ─ 101.022% for quercetin, bisdemethoxycurcumin,demethoxycurcumin and curcumin,respectively, and the inter day accuracy were99.665% ─ 103.06%, 97.669% ─ 103.513%, 99.569% ─103.617%, and 97.929% ─ 103.606%, respectively.
Conclusion: The method was found to be simple, accurateand precise and is recommended for routinequality control analysis of commercial Chinese medicineproducts containing the flour flavonoids as theirprinciple components in the extracts.
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참고문헌 (22)
참고문헌 신청
Ahmed T. / 2009 / Inhibitory effect of curcuminoids on acetylcholinesterase activity and attenuation of scopolamine-ind
Anto RJ. / 1996 / Anti-tumour and free radical scavenging activity of synthetic curcuminoids / Int J Pharm 131 (1) : 1 ~ 7
Barzegar A. / 2012 / The role of electron-transfer and H-atom donation on the superb antioxidant activity and free radical reaction of curcumin / Food Chem 135 (3) : 1369 ~
Bhawana RK. / 2011 / Curcumin nanoparticles : preparation, characterization and antimicrobial study / J Agric Food Chem 59 (5) : 2056 ~ 2061
Gomathi K. / 2003 / Quercetin incorporated collagen matrices for dermal wound healing processes in rat / Biomaterials 24 (16) : 2767 ~ 2772
Anto RJ. / 1996 / Anti-tumour and free radical scavenging activity of synthetic curcuminoids / Int J Pharm 131 (1) : 1 ~ 7
Barzegar A. / 2012 / The role of electron-transfer and H-atom donation on the superb antioxidant activity and free radical reaction of curcumin / Food Chem 135 (3) : 1369 ~
Bhawana RK. / 2011 / Curcumin nanoparticles : preparation, characterization and antimicrobial study / J Agric Food Chem 59 (5) : 2056 ~ 2061
Gomathi K. / 2003 / Quercetin incorporated collagen matrices for dermal wound healing processes in rat / Biomaterials 24 (16) : 2767 ~ 2772
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