메뉴 건너뛰기
.. 내서재 .. 알림
소속 기관/학교 인증
인증하면 논문, 학술자료 등을  무료로 열람할 수 있어요.
한국대학교, 누리자동차, 시립도서관 등 나의 기관을 확인해보세요
(국내 대학 90% 이상 구독 중)
로그인 회원가입 고객센터 ENG
주제분류

추천
검색

논문 기본 정보

자료유형
학술저널
저자정보
저널정보
대한약침학회 Journal of Pharmacopuncture Journal of Pharmacopuncture 제4권 제2호
발행연도
2001.1
수록면
5 - 15 (11page)

이용수

표지
📌
연구주제
📖
연구배경
🔬
연구방법
🏆
연구결과
AI에게 요청하기
추천
검색

초록· 키워드

오류제보하기
This study was carried out for production of neutral antibody to bee venom(anti-phospholipase A2 IgY). Hen layings were injected repeatedly with bee venom and phospholipase A2 with Freund's adjuvant. Specific antibody in egg yolk from immunized hen laying was separated, and purified, also immunological characteristics of anti-phospholipase A2 IgY was invested. The results were summarized as follows :1. Phospholipase A2 was showed single band at molecular weight 17,000 in SDS-PAGE and bee venom was showed two band at molecular weight 17,000 and under molecular weight 6,500 in SDS-PAGE. 2. During 70 days after hen immunized with bee venom and phospholipase A2, antibodies(anti-bee venom IgY) to bee venom were showed poor ELISA value in egg yolk, but antibodies(anti-Phospholipase A2 IgY) to phospholipase A2 in egg yolk were increased ELISA value from 8 days or 15 days and found maximum ELISA value at 42 days. Also after booster at 49 days, ELISA value of anti-Phospholipase A2 IgY in egg yolk was supported at optical density(O.D) 1.0 level, continuously. 3. Titer of phospholipase A2 IgY was showed 1: 32,000. 4. In double immunodiffusion test to phospholipase A2 after double dilution of anti-phospholipase A2 IgY, only precipitation line was made in 1:1 dilution well of anti-Phospholipase A2 IgY. But In immunodiffusion test to anti-phospholipase A2 IgY after double dilution of phospholipase A2, Precipitation line to 250ul/ml well of phospholipase A2 was showed. In double immunodiffusion test to bee venom(1mg/ml) after double dilution anti-phospholipase A2 IgY, all well without 1:32 dilution well were showed strong precipitation line. 5. In dot bloting test to anti-phospholipase A2 IgY after diluting bee venom(0.5mg/ml), dot bloting color was showed clearly to 1/100(5㎍/㎖) in bee venom.

목차

등록된 정보가 없습니다.

참고문헌 (0)

참고문헌 신청

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!

이 논문의 저자 정보

최근 본 자료

전체보기

댓글(0)

0