지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 60 - 66 (7page)
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초록· 키워드
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Background: Enterococcus faecium has emerged as an important nosocomial pathogen worldwide, and this trend has been associated with the dissemination of a genetic lineage designated clonal complex 17 (CC17). In the present study, characterization of the glycopeptide resistance mechanism, genetic relatedness, and pathogenicity in isolates of vancomycin-resistant E. faecium in the Chungcheong area were investigated. Methods: A total of 37 consecutive, non-duplicate, vancomycin-resistant E. faecium were isolated at three university hospitals in the Chungcheong area. The mechanism of glycopeptide resistance and pathogenicity factors were studied using PCR, and the genetic relatedness was determined via multilocus sequence type and esp repeat profile analysis. Additionally, the quinolone resistance-determining regions of parC and gyrA were sequenced to identify mutations involved in ciprofloxacin resistance. Results: Two genotypes of VRE were confirmed: VanAphenotype vanA genotype VRE (25 isolates) and VanB-phenotype vanA genotype VRE (12 isolates). MLST analysis revealed five sequence types. A significant result was that ST414 and CNS4 (4-1-1-1-1-1-1) were considered as belonging to CC17. The esp and hyl genes were found in 100% and 86.4% of the isolates, respectively. A total of 37 isolates showed genetic mutations in parC and gyrA. Conclusion: All isolated strains in the present study belonged to one of the CC17 genotypes including ST414 and CNS4 (4-1-1-1-1-1-1), which were not previously detected in Korea. The combination of MLST and the esp gene repeat profiles can be useful for genetic characterization of VREF isolates with regard to the evolutionary process and epidemiology of the clones.
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참고문헌 (23)
참고문헌 신청
Arthur M / 1993 / Genetics and mechanisms of glycopeptide resistance in enterococci / Antimicrob Agents Chemother 37 : 1563 ~ 1571
Choi YH / 2001 / Molecular relationship of vanA glycopeptide resistance gene in enterococci from hospitalized patients and poultry in Korea / Korean J Infect Dis 33 : 383 ~ 3
Clinical and Laboratory Standards Institute / 2008 / Performance standards for antimicrobial susceptibility testing; eighteenth informational supplement. M100-S18 (M2) / Clinical and L
Eom JS / 2004 / Emergence of vanA genotype vancomycin-resistant enterococci with low or moderate levels of teicoplanin resistance in Korea / J Clin Microbiol 42 : 1785 ~ 178
Homan WL / 2002 / Multilocus sequence typing scheme for Enterococcus faecium / J Clin Microbiol 40 : 1963 ~ 1971
Choi YH / 2001 / Molecular relationship of vanA glycopeptide resistance gene in enterococci from hospitalized patients and poultry in Korea / Korean J Infect Dis 33 : 383 ~ 3
Clinical and Laboratory Standards Institute / 2008 / Performance standards for antimicrobial susceptibility testing; eighteenth informational supplement. M100-S18 (M2) / Clinical and L
Eom JS / 2004 / Emergence of vanA genotype vancomycin-resistant enterococci with low or moderate levels of teicoplanin resistance in Korea / J Clin Microbiol 42 : 1785 ~ 178
Homan WL / 2002 / Multilocus sequence typing scheme for Enterococcus faecium / J Clin Microbiol 40 : 1963 ~ 1971
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