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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 67 - 73 (7page)
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초록· 키워드
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Background: In the present study, the resistance mechanisms against carbapenems and aminoglycosides for 23 strains of multi-drug-resistant Acinetobacter baumannii isolated at a university hospital were investigated.
Methods: The minimal inhibitory concentrations (MICs) were determined via broth microdilution or Etest. The genes encoding OXA-type carbapenemases and 16S rRNA methylase were identified using multiplex PCR, and the amplified products were sequenced. Conjugation experiments were conducted, and an epidemiologic study was performed using enterobacterial repetitive intergenic consensus (ERIC)-PCR. Results: In the isolates, the MICs of the tested aminoglycosides, including arbekacin, were ＞1024 μg/mL; the MICs of aztreonam, cefepime, ceftazidime, and ciprofloxacin ranged from 64 to 128 μg/mL; and the MICs of carbapenem ranged from 32 to 64 μg/mL, as determined through the broth microdilution test. According to the E-test, the MICs of ampicillin/sulbactam and colistin were 8 and 0.25 to 0.38 μg/mL, respectively. Sequence analysis confirmed that all of the isolates expressed carbapenemases OXA-23 and OXA-66, as well as armA 16S rRNA methylase. In addition, ISAba1 was identified upstream of the gene encoding OXA-23. OXA-23 and armA were not transferred to Escherichia coli J53 cells in the transconjugation experiments. ERIC-PCR molecular fingerprinting produced a single pattern in all cases. Conclusion: The co-production of OXA-23 and armA 16S rRNA methylase may be attributed to the multidrug resistance of the A. baumannii isolates in the present study. Stricter surveillance and more rapid detection are necessary to prevent the spread of this type of resistance in the future.
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참고문헌 (21)
참고문헌 신청
[학술
Bogaerts P / 2007 / Emergence of ArmA and RmtB aminoglycoside resistance 16S rRNA methylases in Belgium / J Antimicrob Chemother 59 : 459 ~ 464
Clinical and Laboratory Standards Institute / 2007 / Performance standards for antimicrobial susceptibility testing: seventeenth informational supplement (M100-S17) / CLSI
Doi Y / 2004 / Plasmid-mediated 16S rRNA methylase in Serratia marcescens conferring high-level resistance to aminoglycosides / Antimicrob Agents Chemother 48 : 491 ~ 496
Doi Y / 2007 / Coproduction of novel 16S rRNA methylase RmtD and metallo-β-lactamase SPM-1 in a panresistant Pseudomonas aeruginosa isolate from Brazil / Antimicrob Agents
Bogaerts P / 2007 / Emergence of ArmA and RmtB aminoglycoside resistance 16S rRNA methylases in Belgium / J Antimicrob Chemother 59 : 459 ~ 464
Clinical and Laboratory Standards Institute / 2007 / Performance standards for antimicrobial susceptibility testing: seventeenth informational supplement (M100-S17) / CLSI
Doi Y / 2004 / Plasmid-mediated 16S rRNA methylase in Serratia marcescens conferring high-level resistance to aminoglycosides / Antimicrob Agents Chemother 48 : 491 ~ 496
Doi Y / 2007 / Coproduction of novel 16S rRNA methylase RmtD and metallo-β-lactamase SPM-1 in a panresistant Pseudomonas aeruginosa isolate from Brazil / Antimicrob Agents
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