Objective : Lycii Fructus Extracts(LFE) can do Anti-hypertension activity, Antidepressant, Anti-diabetic activity. Thisstudy was designed to investigate effects of LFE on skin whitening and elasticity using melanoma cells.
Methods : In this experiment, effect of LFE on cell viability, inhibition of melanin synthesis and inhibitory effect ontyrosinase and elastase.
Results : More than 250 ㎍/㎖ of LFE treated group showed lowered proliferation rates significantly compared tonon-treated group. More than 125 ㎍/㎖ of LFE treated groups were lower levels of melanin synthesis respectively. LFEshowed inhibitory effect on tyrosinase activities in vitro. And, LFE suppressed tyrosinase activities in B16F10 cellssignificantly. Finally, LFE suppressed elastase type Ⅰ and Ⅳ activities in dose-dependent manner in vitro. And LFE alsoslightly suppressed elastase activities in vivo.
Conclusion : These results suggest that LFE can inhibit melanin synthesis through ihhibitory action on tyrosinaseactivity and inhibt elastase activity, and also suggest that these results can be used for the study on maintaining skinelasticity or whitening.