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논문 기본 정보

자료유형
학술저널
저자정보
저널정보
대한한방안이비인후피부과학회 한방안이비인후피부과학회지 한방안이비인후피부과학회지 제26권 제1호
발행연도
2013.1
수록면
19 - 34 (16page)

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Objective : This study was performed to evaluate the effect of immune reaction inductive substances such as PMA, LPS, DPE, DNCB and WP, the whitman pigra extracting substance at simultaneously on the translocation of NFκB towards to the nucleus and the mRNA expression patterns of various cytokine genes in THP-1 cells,monocytes of human. Methods : To analyze the cytokine genes expressions, the RT-PCR method was used, and measuring TNF-αthat had been secreted during cell culture by the ELISA method. The morphological changes were observed during THP-1 cell by a scanning electron microscope and the quantitative distribution of NFκB in the cell that was analyzed through immunocytochemistry and a confocal microscopy. Results : WP showed different influences onto the mRNA expression patterns of cytokine genes with PMA, LPS. DPE and DNCB according to the types of immune inductive substances in the THP-1 cells. Upon treating PMA and DPE on the THP-1 cells at the same time or either additionally treating WP thereon, the movement of NFκB increase towards the nucleus from cell cytoplasm was able to be observed. The expressions of IL-1α and IFN-γinduced by PMA and PMA+DNCB were suppressed by WP while the expression of TGF-β was promoted. Regarding the secretion pattern of TNF-α according to the treatment of PMA, its secretion amount was incredibly increased by concurrent treatment of WP, however, in case of co-treatment of WP with PMA and DNCB, it was found that the secretion amount of TNF-α decreased. Conclusions : In this study, the WP extracting substance was confirmed that it had an influence on expression patterns of cytokine genes according to the actions of a variety kinds of immune reaction inductive substances treated on the THP-1 cells. Especially, WP co-treatment with PMA and DNCB was suppressed the expression of inflammatory cytokines, such as IL-1α, IFN-γ and TNF-α.
#cytokines #Whitmania pigra #immune respones #THP-1 #TNF-α

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참고문헌 (26)

참고문헌 신청
Abbas AK / 1996 / Functional diversity of helper T lymphocytes / Nature 383 : 787 ~ 793 google schola Anjana Rao / 2000 / Molecular aspects of T-cell differentiation / British Medical Bulletin 56 : 969 ~ 984 google schola Cetkovic-Cvrlje M / 1994 / TNF and IFN γ potentiate the deleterious effects of IL-1β on mouse pancreatic islets mainly via generation of nitric oxide / Cytokine 6 : 399 ~ 4 google schola Chan SC / 1993 / Altered prostaglandin E2 regulation of cytokine production in atopic dermatitis / J Immunol 151 : 3345 ~ 3352 google schola Charles A / 2002 / Innate immune recognition / Annu Rev Immunol 20 : 197 ~ 216 google schola

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