지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 746 - 752 (7page)
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초록· 키워드
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Purpose: Tuberculosis (TB) is a major infectious disease and is responsible for two million deaths annually. For the identification and quantitation of Mycobacteriumtuberculosis (M. tuberculosis), a causative agent of TB, a sandwich enzyme-linked immunosorbent assay (ELISA) against the MPT64 protein of M. tuberculosis,an antigen marker of the M. tuberculosis complex, was developed. Materials and Methods: The MPT64 protein was expressed, and anti-MPT64 monoclonal antibodies were prepared. A sandwich ELISA was established using recombinant MPT64 protein and anti-MPT64 monoclonal antibodies. The sandwich MPT64 ELISA was evaluated using reference and clinical mycobacterial strains. Results: The sandwich MPT64 ELISA detected MPT64 protein from 2.1 ng/mL to 250 ng/mL (equivalent to 1.7×104 CFU/mL and 2.0×106 CFU/mL). All 389 clinical M. tuberculosisisolates tested positive in the sandwich MPT64 ELISA (sensitivity, 100%), and the assay showed no cross reactivity to any tested nontuberculous mycobacterialstrain (specificity, 100%). Conclusion: The sandwich MPT64 ELISA is a highly sensitive and quantitative test for MPT64 protein, which can identify M. tuberculosis.
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참고문헌 (16)
참고문헌 신청
Ang CF / 2011 / Evaluation of a rapid assay for identification of Mycobacterium tuberculosis grown in solid and liquid media / Int J Tuberc Lung Dis 15 : 1475 ~ 1477
Brent AJ / 2011 / Performance of the MGIT TBc identification test and meta-analysis of MPT64 assays for identification of the Mycobacterium tuberculosis complex in liquid cu
Chakravorty S / 2005 / Diagnosis of extrapulmonary tuberculosis by smear, culture, and PCR using universal sample processing technology / J Clin Microbiol 43 : 4357 ~ 4362
Cho SN / 2007 / Tuberculosis: diagnostics / Tuberculosis (Edinb) 87 (Suppl 1) : S14 ~ S17
Hasegawa N / 2002 / New simple and rapid test for culture confirmation of Mycobacterium tuberculosis complex: a multicenter study / J Clin Microbiol 40 : 908 ~ 912
Brent AJ / 2011 / Performance of the MGIT TBc identification test and meta-analysis of MPT64 assays for identification of the Mycobacterium tuberculosis complex in liquid cu
Chakravorty S / 2005 / Diagnosis of extrapulmonary tuberculosis by smear, culture, and PCR using universal sample processing technology / J Clin Microbiol 43 : 4357 ~ 4362
Cho SN / 2007 / Tuberculosis: diagnostics / Tuberculosis (Edinb) 87 (Suppl 1) : S14 ~ S17
Hasegawa N / 2002 / New simple and rapid test for culture confirmation of Mycobacterium tuberculosis complex: a multicenter study / J Clin Microbiol 40 : 908 ~ 912
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