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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 324 - 330 (7page)
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Purpose: This study was conducted to investigate the small double-stranded RNA (dsRNA) mediated anti-tumor effects of p21WAF1/ClP1 (p21) transcriptional activation in vitro in the human glioma SHG-44 cell line. Materials and Methods: Human glioma SHG-44 cells were transfected with dsRNA using LipofectAMINE 2000 transfection reagent. Real-time PCR and Western blot analysis were conducted to detect p21 and survivin mRNA and protein levels, respectively. Cell proliferation was examined by MTT assay. Cell cycle distribution and apoptosis were detected by flow-cytometric analysis. Results: We found that dsRNA targeting p21 promoter (dsP21) significantly induced the expression of p21 at transcription and protein levels,and reduced the expression of survivin. AS well, dsP21 transcription significantlyinhibited human glioma SHG-44 cell proliferation. Analysis of cell cycle distributionrevealed that dsP21 transfection increased accumulation of cells in the G0/G1 phase and reduced accumulation of cells in the S phase. Further analysis revealed that dsP21 transcription led to an increase in both early and late stages of apoptosis in human glioma SHG-44 cells. Conclusion: In the present study, P21 activation by RNA-induced gene activation (RNAa) induced anti-tumor activity in vitro in a humanglioma SHG-44 cell line. The results suggested that RNAa could be used for human glioma treatment by targeted activation of tumor suppressor genes.
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참고문헌 (30)
참고문헌 신청
Besson A / 2004 / Regulation of the cytoskeleton: an oncogenic function for CDK inhibitors? / Nat Rev Cancer 4:948~955
Wang J / 2010 / Prognostic value and function of KLF4 in prostate cancer: RNAa and vector-mediated overexpression identify KLF4 as an inhibitor of tumor cell growth and migration / Cancer Res 70:10182~10191
Matsui M / 2010 / Activation of LDL receptor expression by small RNAs complementary to a noncoding transcript that overlaps the LDLR promoter / Chem Biol 17:1344~1355
Chen R / 2011 / Up-regulation of VEGF by small activator RNA in human corpus cavernosum smooth muscle cells / J Sex Med 8:2773~2780
Chu Y / 2010 / Involvement of argonaute proteins in gene silencing and activation by RNAs complementary to a non-coding transcript at the progesterone receptor promoter / Nucleic Acids Res 38:7736~7748
Wang J / 2010 / Prognostic value and function of KLF4 in prostate cancer: RNAa and vector-mediated overexpression identify KLF4 as an inhibitor of tumor cell growth and migration / Cancer Res 70:10182~10191
Matsui M / 2010 / Activation of LDL receptor expression by small RNAs complementary to a noncoding transcript that overlaps the LDLR promoter / Chem Biol 17:1344~1355
Chen R / 2011 / Up-regulation of VEGF by small activator RNA in human corpus cavernosum smooth muscle cells / J Sex Med 8:2773~2780
Chu Y / 2010 / Involvement of argonaute proteins in gene silencing and activation by RNAs complementary to a non-coding transcript at the progesterone receptor promoter / Nucleic Acids Res 38:7736~7748
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