지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 1,130 - 1,137 (8page)
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Purpose: To determine whether renal injury induced by ischemia-reperfusion (I/R) could be further improved by mesenchymal stem cells (MSCs) modified with survivin. Materials and Methods: Lentiviral vectors were used to introduce the survivingene into MSCs and the MSCs modified with survivin were transplanted into established mice models of renal I/R injury. Seven days later, serum creatinine (Scr) and blood urea nitrogen (BUN) were measured and the survival of MSCs was determined. Hematoxylin and eosin staining was used to assess renal pathologicalchange. The expressions of hepatocyte growth factor (HGF) and basic fibroblastgrowth factor (bFGF) in kidney tissue were detected by western blot. Results:Mice transplanted with survivin-modified MSCs demonstrated good renal function recovery with Scr and BUN decline close to normal levels and improvementof renal I/R injury repair. Additionally, the survival of transplanted MSCs modified with survivin was enhanced and the expression of HGF and bFGF in kidney tissue was increased. Conclusion: Our results demonstrated that MSCs engineeredto over-express survivin could enhance their therapeutic effect on renal I/R injury in mice, probably via the improved survival ability of MSCs and increasedproduction of protective cytokines in ischemic tissue.
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참고문헌 (23)
참고문헌 신청
Chen J / 2002 / Caspase inhibition by Z-VAD increases the survival of grafted bone marrow cells and improves functional outcome after MCAo in rats / J Neurol Sci 199 : 17 ~
Chiou SK / 2003 / Survivin - an anti-apoptosis protein: its biological roles and implications for cancer and beyond / Med Sci Monit 9 : PI25 ~ PI29
Chua HR / 2012 / Acute kidney injury after cardiac arrest / Resuscitation 83 : 721 ~ 727
Deng W / 2003 / Adenoviral gene transfer of eNOS: high-level expression in ex vivo expanded marrow stromal cells / Am J Physiol Cell Physiol 285 : C1322 ~ C1329
Duffield JS / 2005 / Kidney tubular epithelium is restored without replacement with bone marrow-derived cells during repair after ischemic injury / Kidney Int 68 : 1956 ~ 19
Chiou SK / 2003 / Survivin - an anti-apoptosis protein: its biological roles and implications for cancer and beyond / Med Sci Monit 9 : PI25 ~ PI29
Chua HR / 2012 / Acute kidney injury after cardiac arrest / Resuscitation 83 : 721 ~ 727
Deng W / 2003 / Adenoviral gene transfer of eNOS: high-level expression in ex vivo expanded marrow stromal cells / Am J Physiol Cell Physiol 285 : C1322 ~ C1329
Duffield JS / 2005 / Kidney tubular epithelium is restored without replacement with bone marrow-derived cells during repair after ischemic injury / Kidney Int 68 : 1956 ~ 19
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