지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 999 - 1,004 (6page)
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Purpose: House dust mites (HDMs) are an important source of indoor allergens associated with asthma, rhinitis and atopic dermatitis. Chicken immunoglobulin (Ig) Y is known to be a good alternative to mice and rabbit antibody production. In this study, we produced IgYs specific to HDMs and investigated their IgE immunoreactivities. Materials and Methods: Total IgYs were isolated from the yolks of White Leghorn hens immunized with either Dermatophagoides pteronyssinus or D. farinae protein extract. Control antibodies were separated from the yolks of immunizedhens with phosphate buffered saline. IgYs specific to HDMs were analyzedusing enzyme-linked immunosorbent assay and Western blotting analysis. Results: The concentration of egg IgY specific to D. farinae in an immunized hen increased and the highest achieved was 661.3 ug/mg (per an egg) on day 47, comparedwith 760 ug/mg IgY specific to D. pteronyssinus on day 16. The D. pteronyssinusor D. farinae-specific IgY was detected by binding of each mite proteins, and their immunoreactivities were elevated dependent of the specific IgY concentration. Conclusion: IgY specific to HDMs may be a promising antibody for immunologicaldiagnosis as well as identification of possible resistance relating to HDM allergy.
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참고문헌 (25)
참고문헌 신청
Asturias JA / 2009 / Engineering of major house dust mite allergens Der p 1 and Der p 2 for allergen-specific immunotherapy / Clin Exp Allergy 39 : 1088 ~ 1098
Casadevall A / 1995 / Antibody immunity and invasive fungal infections / Infect Immun 63 : 4211 ~ 4218
Chalghoumi R / 2008 / Production of hen egg yolk immunoglobulins simultaneously directed against Salmonella enteritidis and Salmonella typhimurium in the same egg yolk / Pou
Chua KY / 1990 / Isolation of cDNA coding for the major mite allergen Der p II by IgE plaque immunoassay / Int Arch Allergy Appl Immunol 91 : 118 ~ 123
Dias da Silva W / 2010 / IgY: a promising antibody for use in immunodiagnostic and in immunotherapy / Vet Immunol Immunopathol 135 : 173 ~ 180
Casadevall A / 1995 / Antibody immunity and invasive fungal infections / Infect Immun 63 : 4211 ~ 4218
Chalghoumi R / 2008 / Production of hen egg yolk immunoglobulins simultaneously directed against Salmonella enteritidis and Salmonella typhimurium in the same egg yolk / Pou
Chua KY / 1990 / Isolation of cDNA coding for the major mite allergen Der p II by IgE plaque immunoassay / Int Arch Allergy Appl Immunol 91 : 118 ~ 123
Dias da Silva W / 2010 / IgY: a promising antibody for use in immunodiagnostic and in immunotherapy / Vet Immunol Immunopathol 135 : 173 ~ 180
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