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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 1,005 - 1,013 (9page)
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Purpose: To evaluate a multi-group-specific sequence-based typing (SBT) method for resolving ambiguous results from human leukocyte antigen (HLA) genotyping. Materials and Methods: A total of 50 samples that showed ambiguous genotypes for at least two HLA loci from HLA-A, -B, -C and -DRB1 by the conventional SBT assay were evaluated using a new SBT test, the AVITA plus assay. The most likely HLA genotypes for the respective samples considering allele frequencies in Korean were concordant between the AVITA and conventional SBT assays. Results: An averageof 3.3 loci among the HLA-A, -B, -C and -DRB1 loci per sample gave results with two or more possible allele combinations with the conventional SBT, and 48 (96.0%) out of 50 showed reduced numbers of possible genotypes for at least one HLA locus with the AVITA. A total of 41, 43, 42, and 38 cases among the 50 samples showed ambiguous results for HLA-A, -B, -C, and -DRB1 typing by the conventional SBT, respectively. The average numbers of possible allele combinations for the respectivefour HLA loci were 8.2, 6.7, 5.9, and 3.2, and they were reduced to 1.5, 2.2, 4.4, and 1.8, respectively, by the AVITA. Ambiguity was resolved by the AVITA in 33 (80.5%), 31 (72.1%), 17 (40.5%) and 28 (73.7%) samples among the ambiguous cases from the conventional SBT for HLA-A, -B, -C, and -DRB1 typing, respectively. Conclusion: The multi-group-specific SBT method considerably reduced the numberof ambiguous results, and thus may be useful for accurate HLA typing in clinical laboratories.
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참고문헌 (20)
참고문헌 신청
Adams SD / 2004 / Ambiguous allele combinations in HLA Class I and Class II sequence-based typing: when precise nucleotide sequencing leads to imprecise allele identification
Cano P / 2007 / Common and well-documented HLA alleles: report of the Ad-Hoc committee of the american society for histocompatiblity and immunogenetics / Hum Immunol 68 : 392
Dapprich J / 2008 / SNP-specific extraction of haplotype-resolved targeted genomic regions / Nucleic Acids Res 36 : e94 ~
Elsner HA / 2004 / Immunogenetics of HLA null alleles: implications for blood stem cell transplantation / Tissue Antigens 64 : 687 ~ 695
Erlich H / 2012 / HLA DNA typing: past, present, and future / Tissue Antigens 80 : 1 ~ 11
Cano P / 2007 / Common and well-documented HLA alleles: report of the Ad-Hoc committee of the american society for histocompatiblity and immunogenetics / Hum Immunol 68 : 392
Dapprich J / 2008 / SNP-specific extraction of haplotype-resolved targeted genomic regions / Nucleic Acids Res 36 : e94 ~
Elsner HA / 2004 / Immunogenetics of HLA null alleles: implications for blood stem cell transplantation / Tissue Antigens 64 : 687 ~ 695
Erlich H / 2012 / HLA DNA typing: past, present, and future / Tissue Antigens 80 : 1 ~ 11
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