지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 679 - 685 (7page)
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Purpose: If cholesterol in the cell membrane is depleted by treating cells with methyl-β-cyclodextrin (MβCD), the activities of transmembrane receptors are altered in a cell-specific and/or receptor-specific manner. The proinflammatory cytokines,IL-1β is potent inducers of MUC5AC mRNA and protein synthesis in human airway epithelial cells. Cells activated by IL-1β showed increased phosphorylation of extracellular signal regulated kinase (ERK) and p38 mitogen-activated protein kinase (MAPK). Thus, we investigated the effects of cholesterol depletion on the expression of MUC5AC in human airway epithelial cells and whether these alterations to MUC5AC expression were related to MAPK activity. Materials and Methods: After NCI-H292 cells were pretreated with 1% MβCD before adding IL-1β for 24 hours, MUC5AC mRNA expression was determined by reverse transcription-polymerase chain reaction (RT-PCR) and real time-PCR. Cholesterol depletion by MβCD was measured by modified microenzymatic fluorescence assay and filipin staining. The phosphorylation of IL-1 receptor, ERK and p38 MAPK, was analyzed by western blot. Results: Cholesterol in the cell membrane was significantly depleted by treatment with MβCD on cells. IL-1β-induced MUC5AC mRNA expression was decreased by MβCD and this decrease occurred IL-1-receptor-specifically. Moreover, we have shown that MβCD suppressed the activation of ERK1/2 and p38 MAPK in cells activated with IL-1β. This result suggests that MβCD-mediated suppression of IL-1β-induced MUC5AC mRNA operated via the ERK- and p38 MAPK-dependent pathway. Conclusion: Cholesterol depletion in NCI-H292 cell membrane may be considered an anti-hypersecretory method since it effectively inhibits mucus secretion of respiratory epithelial cells.
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참고문헌 (24)
참고문헌 신청
Bang B / 2005 / Disruption of lipid rafts causes apoptotic cell death in HaCaT keratinocytes / Exp Dermatol 14 : 266 ~ 272
Brown DA / 1998 / Functions of lipid rafts in biological membranes / Annu Rev Cell Dev Biol 14 : 111 ~ 136
Burger K / 2000 / Regulation of receptor function by cholesterol / Cell Mol Life Sci 57 : 1577 ~ 1592
Calleros L / 2006 / Low cell cholesterol levels increase NFkappaB activity through a p38 MAPK-dependent mechanism / Cell Signal 18 : 2292 ~ 2301
Chen X / 2002 / Cholesterol depletion from the plasma membrane triggers ligand-independent activation of the epidermal growth factor receptor / J Biol Chem 277 : 49631 ~ 496
Brown DA / 1998 / Functions of lipid rafts in biological membranes / Annu Rev Cell Dev Biol 14 : 111 ~ 136
Burger K / 2000 / Regulation of receptor function by cholesterol / Cell Mol Life Sci 57 : 1577 ~ 1592
Calleros L / 2006 / Low cell cholesterol levels increase NFkappaB activity through a p38 MAPK-dependent mechanism / Cell Signal 18 : 2292 ~ 2301
Chen X / 2002 / Cholesterol depletion from the plasma membrane triggers ligand-independent activation of the epidermal growth factor receptor / J Biol Chem 277 : 49631 ~ 496
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