지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 1,036 - 1,044 (9page)
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Purpose: The aim of this study was to identify the adipocyte-specific gene expression patterns in chorion-derived mesenchymal stem cells during adipogenic differentiation.
Materials and Methods: Chorionic cells were isolated from the third trimester chorions from human placenta at birth and identified morphologically and by fluorescence-activated cell sorting analysis. After inducing adipogenic differentiation for 28 days, cells at days 3, 10, 21 and 28 were analyzed by Oil red O staining and RNA extraction in order to assess the expression levels of adipocyte marker genes, including CCAAT-enhancer binding protein α (C/EBPα), peroxisome proliferator-activated receptor γ (PPARγ), fatty acid binding protein 4 (FABP4) and Glycerol-3-phosphate dehydrogenase (GPD2). Cells not induced for differentiation were compared with the induced cells as a control group. Results: Chorion-derived cells showed the same pattern as fibroblasts, and expressed CD73, CD105, and CD166 antigens, but not CD45, CD34, and HLA-DR antigens. On day 3 after differentiation,cells began to stain positively upon Oil red O staining, and continuously increased in lipid granules for 4 weeks. The expression level of C/EBPα increased 4.6 fold on day 3 after induction, and continued to increase for 4 weeks. PPARγ was expressed at a maximum of 2.9 fold on day 21. FABP4 and GPD2 were significantly expressed at 4.7- and 3.0-fold, respectively, on day 21, compared to controls, and further increased thereafter. Conclusion: Human chorion-derived mesenchymal stem cells exhibited the sequential expression pattern of adipocyte marker genes during differentiation, corresponding to adipogenesis.
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참고문헌 (31)
참고문헌 신청
Auwerx J / 1996 / Transcription, adipocyte differentiation, and obesity / J Mol Med 74:347~352
Shin DW / 2009 / (-)-Catechin promotes adipocyte differentiation in human bone marrow mesenchymal stem cells through PPAR gamma transactivation / Biochem Pharmacol 77:125~133
Chang YJ / 2006 / Disparate mesenchyme-lineage tendencies in mesenchymal stem cells from human bone marrow and umbilical cord blood / Stem Cells 24:679~685
Karahuseyinoglu S / 2008 / Functional structure of adipocytes differentiated from human umbilical cord stroma-derived stem cells / Stem Cells 26:682~691
Sekiya I / 2004 / Adipogenic differentiation of human adult stem cells from bone marrow stroma (MSCs) / J Bone Miner Res 19:256~264
Shin DW / 2009 / (-)-Catechin promotes adipocyte differentiation in human bone marrow mesenchymal stem cells through PPAR gamma transactivation / Biochem Pharmacol 77:125~133
Chang YJ / 2006 / Disparate mesenchyme-lineage tendencies in mesenchymal stem cells from human bone marrow and umbilical cord blood / Stem Cells 24:679~685
Karahuseyinoglu S / 2008 / Functional structure of adipocytes differentiated from human umbilical cord stroma-derived stem cells / Stem Cells 26:682~691
Sekiya I / 2004 / Adipogenic differentiation of human adult stem cells from bone marrow stroma (MSCs) / J Bone Miner Res 19:256~264
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