지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 999 - 1,007 (9page)
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초록· 키워드
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Purpose: This study examined a rapid isolation method decreasing the time and cost of the clinical application of adipose tissue-derived stem cells (ASCs). Materials and Methods: Aliquots (10 g) of the lipoaspirates were stored at 4°C without supplying oxygen or nutrients. At the indicated time points, the yield of mononuclear cells was evaluated and the stem cell population was counted by colony forming unit-fibroblast assays. Cell surface markers, stem cell-related transcription factors, and differentiation potentials of ASCs were analyzed. Results: When the lipoaspirates were stored at 4°C, the total yield of mononuclear cells decreased, but the stem cell population was enriched. These ASCs expressed CD44, CD73, CD90, CD105, and HLA-ABC but not CD14, CD31, CD34, CD45, CD117, CD133, and HLA-DR. The number of ASCs increased 1×1014 fold for 120 days. ASCs differentiated into osteoblasts, adipocytes, muscle cells, or neuronal cells. Conclusion: ASCs isolated from lipoaspirates and stored for 24 hours at 4°C have similar properties to ASCs isolated from fresh lipoaspirates. Our results suggest that ASCs can be isolated with high frequency by optimal storage at 4°C for 24 hours, and those ASCs are highly proliferative and multipotent, similar to ASCs isolated from fresh lipoaspirates. These ASCs can be useful for clinical application because they are time- and cost-efficient, and these cells maintain their stemness for a long time, like ASCs isolated from fresh lipoaspirates.
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참고문헌 (25)
참고문헌 신청
Campagnoli C / 2001 / Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood, liver, and bone marrow / Blood 98 : 2396 ~ 2402
Colter DC / 2000 / Rapid expansion of recycling stem cells in cultures of plastic-adherent cells from human bone marrow / Proc Natl Acad Sci U S A 97 : 3213 ~ 3218
De Ugarte DA / 2003 / Comparison of multi-lineage cells from human adipose tissue and bone marrow / Cells Tissues Organs 174 : 101 ~ 109
Erices A / 2000 / Mesenchymal progenitor cells in human umbilical cord blood / Br J Haematol 109 : 235 ~ 242
Friedenstein AJ / 1968 / Heterotopic of bone marrow. Analysis of precursor cells for osteogenic and hematopoietic tissues / Transplantation 6 : 230 ~ 247
Colter DC / 2000 / Rapid expansion of recycling stem cells in cultures of plastic-adherent cells from human bone marrow / Proc Natl Acad Sci U S A 97 : 3213 ~ 3218
De Ugarte DA / 2003 / Comparison of multi-lineage cells from human adipose tissue and bone marrow / Cells Tissues Organs 174 : 101 ~ 109
Erices A / 2000 / Mesenchymal progenitor cells in human umbilical cord blood / Br J Haematol 109 : 235 ~ 242
Friedenstein AJ / 1968 / Heterotopic of bone marrow. Analysis of precursor cells for osteogenic and hematopoietic tissues / Transplantation 6 : 230 ~ 247
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