지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 187 - 196 (10page)
이용수
초록· 키워드
오류제보하기
Purpose: Ca2+ homeostasis plays an important role in myocardial cell injury induced by hypoxia-reoxygenation, and prevention of intracellular Ca2+ overload is key to cardioprotection. Even though thiopental is a frequently used anesthetic agent, little is known about its cardioprotective effects, particulary in association with Ca2+ homeostasis.
We investigated whether thiopental protects cardiomyocytes against hypoxia-reoxygenation injury by regulating Ca2+homeostasis. Materials and Methods: Neonatal rat cardiomyocytes were isolated. Cardiomyocytes were exposed to different concentrations of thiopental and immediately replaced in the hypoxic chamber to maintain hypoxia. After 1hour of exposure, a culture dish was transferred to the CO2 incubator and cells were incubated at 37°C for 5 hours. At the end of the experiments, the authors assessed cell protection using immunoblot analysis and caspase activity. The mRNA of genes involved in Ca2+ homeostasis, mitochondrial membrane potential, and cellular Ca2+ levels were examined. Results: In thiopental-treated cardiomyocytes, there was a decrease in expression of the proapoptotic protein Bax, caspase-3 activation, and intracellular Ca2+ content. In addition, both enhancement of anti-apoptotic protein Bcl-2 and activation of Erk concerned with survival were shown. Furthermore, thiopental attenuated alterations of genes involving Ca2+ regulation and significantly modulated abnormal changes of NCX and SERCA2a genes in hypoxia-reoxygenated neonatal cardiomyocytes. Thiopental suppressed disruption of mitochondrial membrane potential (ΔΨm) induced by hypoxia-reoxygenation. Conclusion: Thiopental is likely to modulate expression of genes that regulate Ca2+ homeostasis, which reduces apoptotic cell death and results in cardioprotection.
목차
등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
[학술지
Almaas R / 2000 / Effect of barbiturates on hydroxyl radicals, lipid peroxidation, and hypoxic cell death in human NT2-N neurons / Anesthesiology 92 : 764 ~ 774
Anaya-Prado R / 2002 / The molecular events underlying ischemia/reperfusion injury / Transplant Proc 34 : 2518 ~ 2519
Cossarizza A / 1993 / A new method for the cytofluorimetric analysis of mitochondrial membrane potential using the J-aggregate forming lipophillic cation 5,5’,6,6’-tetrach
Fliss H / 1996 / Apoptosis in ischemic and reperfused rat myocardium / Circ Res 79 : 949 ~ 956
Almaas R / 2000 / Effect of barbiturates on hydroxyl radicals, lipid peroxidation, and hypoxic cell death in human NT2-N neurons / Anesthesiology 92 : 764 ~ 774
Anaya-Prado R / 2002 / The molecular events underlying ischemia/reperfusion injury / Transplant Proc 34 : 2518 ~ 2519
Cossarizza A / 1993 / A new method for the cytofluorimetric analysis of mitochondrial membrane potential using the J-aggregate forming lipophillic cation 5,5’,6,6’-tetrach
Fliss H / 1996 / Apoptosis in ischemic and reperfused rat myocardium / Circ Res 79 : 949 ~ 956
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Microarray analysis of gene expression induced by hypoxia and doxorubicin in cardiomyocytes
환경독성보건학회 심포지엄 및 학술대회
2006 .11
-
Changes in drug metabolism during hypoxia/reoxygenation in isolated perfused rat
한국응용약물학회 춘계학술발표논문집
1997 .01
-
저산소 모델에 따른 간장 기능 손상에 관한 연구
The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmacology
2000 .01
-
흰쥐 관류간 모델에서 저산소 및 산소재도입시 vitamin C가 간장기능에 미치는 영향
The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmacology
1997 .01
-
골아세포내 $Ca^{2+}$ 활성도의 조절기전
기초간호자연과학회지
1999 .01
-
Controlled Gene Expression System under Hypoxia Conditions
항공우주의학 학술대회
2015 .05
-
Effect of Trolox C on Hypoxia/Reoxygenation-Induced Injury in Isolated Perfused Rat Liver
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
1997 .01
-
Catalase protects cardiomyocytes via its inhibition of nitric oxide synthesis
대한약학회 학술대회
2003 .01
-
Tumor hypoxia and reoxygenation: the yin and yang for radiotherapy
Radiation oncology journal : ROJ
2016 .01
-
Effect of vitamin C on hepatic drug metabolism in hypoxia/reoxygenation
한국응용약물학회 추계학술발표논문집
1998 .01
-
Acetaminophen Induced Cytotoxicity and Altered Gene Expression in Cultured Cardiomyocytes of H9C2 Cells
Environmental Analysis Health and Toxicology
2012 .01
-
Enhancement of Ca Current Does Not Regulate the Speed of Depolarization-induced Ca Propagation Wave in Rat Atrial Myocytes
The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmacology
2007 .01
-
단위발생 유래 생쥐 배아줄기세포의 기능성 심근세포 형성
대한불임학회지
2002 .01
-
The over-expression of RGS19 affects the differentiation of cardiomyocytes
한국실험동물학회 학술발표대회 논문집
2010 .02
-
탈분극과 근장그물 내 $Ca^{2+}$ 고갈-유도 평활근의 수축 및 세포 내 $Ca^{2+}$ 변동에 관여하는 L-형 $Ca^{2+}$ 통로의 상관성
대한물리치료학회지(JKPT)
2007 .01
-
Differentiation of Cardiomyocyte from Human Embryonic Stem Cell
대한생식의학회 학술대회
2004 .01
-
저산소 환경에서의 유전자 발현 조절
항공우주의학회지
2015 .12
-
Hexane Soluble Fraction of Chungpesagan-tang Exhibits Protective Effect against Hypoxia/Reoxygenation-Induced N2a Cell Damage
Biomolecules & Therapeutics(구 응용약물학회지)
2008 .01
-
Protective Effect of Propofol against Hypoxia-reoxygenation Injury in HaCaT Human Keratinocytes
International Journal of Oral Biology
2014 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0