지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 165 - 172 (8page)
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Purpose: Imbalances between osteogenic and adipogenic differentiation leads to diseases such as osteoporosis. The aim of our study was to demonstrate the differences in extracellular signal-regulated kinase (ERK) phosphorylation during both adipogenesis and osteogenesis of human bone marrow-derived stem cells (BMSCs). Materials and Methods: Using troglitazone, GW9662 and U0126, we investigated their role in hBMSC differentiation to adipogenic and osteogenic fates. Results: ERK1/2 inhibition by U0126 suppressed proliferator-activated receptor (PPAR)γ expression and lipid accumulation, while it decreased the mRNA expression of adipogenic genes (lipoprotein lipase, PPARγ, and adipocyte protein) and osteogenic genes (type I collagen and osteopontin). ERK phosphorylation was transient and decreased during adipogenesis, whereas it occurred steadily during osteogenesis. Troglitazone, a PPARγ agonist, induced adipogenesis by inhibiting ERK phosphorylation even in an osteogenic medium, suggesting that ERK signaling needs to be shut off in order to proceed with adipose cell commitment. Cell proliferation was greatly increased in osteogenesis but was not changed during adipogenesis, indicating that ERK might play different roles in cellular proliferation and differentiation between the two committed cell types. Conclusion: The duration and magnitude of ERK activation might be a crucial factor for the balance between adipogenesis and osteogenesis in human bone marrow-derived stem cells.
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참고문헌 (23)
참고문헌 신청
Aubert J / 1999 / Leukemia inhibitory factor and its receptor promote adipocyte differentiation via the mitogen-activated protein kinase cascade / J Biol Chem 274 : 24965 ~
Beresford JN / 1992 / Evidence for an inverse relationship between the differentiation of adipocytic and osteogenic cells in rat marrow stromal cell cultures / J Cell Sci 102
Bost F / 2002 / Retinoic acid activation of the ERK pathway is required for embryonic stem cell commitment into the adipocyte lineage / Biochem J 361 : 621 ~ 627
Burgermeister E / 2007 / MAPK kinases as nucleo-cytoplasmic shuttles for PPARgamma / Cell Cycle 6 : 1539 ~ 1548
Chan GK / 2001 / PTHrP inhibits adipocyte differentiation by down-regulating PPAR gamma activity via a MAPK-dependent pathway / Endocrinology 142 : 4900 ~ 4909
Beresford JN / 1992 / Evidence for an inverse relationship between the differentiation of adipocytic and osteogenic cells in rat marrow stromal cell cultures / J Cell Sci 102
Bost F / 2002 / Retinoic acid activation of the ERK pathway is required for embryonic stem cell commitment into the adipocyte lineage / Biochem J 361 : 621 ~ 627
Burgermeister E / 2007 / MAPK kinases as nucleo-cytoplasmic shuttles for PPARgamma / Cell Cycle 6 : 1539 ~ 1548
Chan GK / 2001 / PTHrP inhibits adipocyte differentiation by down-regulating PPAR gamma activity via a MAPK-dependent pathway / Endocrinology 142 : 4900 ~ 4909
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