지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 25 - 28 (4page)
이용수
초록· 키워드
오류제보하기
Connexin 43 is the most predominant type of connexin expressed on rat detrusor muscle cells. We investigated the connexin 43 expressions in various age groups of either sex in normal rats.
Methods: Eighty Sprague-Dawley rats were used for analysis. Each group was quantified by 8 rats at 1 week, 2 weeks, 1 month, 3 months, and 6 months of age in either sex. In each animal, bladder was removed without any kind of intervention and fresh-frozen in liquid nitrogen. Total RNA extraction was done with easy-BLUE total RNA extraction kit. Reverse transcription polymerase chain reaction was done for connexin 43 and glyceraldehyde-3-phosphate dehydrogenase as an internal control using ImProm-II Reverse Transcription System.
Results: In female rats, no age-related change was detected in connexin 43 expressions. In male rats, connexin expression at 3 months of age showed significant decrease compared with 1 week, 2 weeks, and 6 months of age (P<0.05). When connexin expression at the same age in male and female were compared, only 3 months group in male showed significant decrease than the same age group in female.
Conclusions: Our data suggest that the expressions of connexin 43 mRNA in normal detrusor muscle cell showed age-related changes especially in male rats. Although it is difficult to interpret these findings at this stage, age should be considered as a possible compounding factor affecting connexin 43 expressions in male rats.
목차
등록된 정보가 없습니다.
참고문헌 (15)
참고문헌 신청
Christ GJ / 2003 / Increased connexin43-mediated intercellular communication in a rat model of bladder overactivity in vivo / Am J Physiol Regul Integr Comp Physiol 284 : 124
Chun AL / 1990 / Comparison of uri\-nary bladder function in sexually mature and immature male and female rats / J Urol 143 : 1267 ~ 1271
Fromaget C / 1992 / Distribution pattern of con\-nexin 43, a gap junctional protein, during the differentiation of mouse heart myocytes / Differentiation 51 : 9 ~ 20
Haefliger JA / 2002 / Connexins 43 and 26 are differentially increased after rat bladder outlet obstruction / Exp Cell Res 274 : 216 ~ 225
Haferkamp A / 2004 / Increased expression of connexin 43 in the overactive neuro\-genic detrusor / Eur Urol 46 : 799 ~ 805
Chun AL / 1990 / Comparison of uri\-nary bladder function in sexually mature and immature male and female rats / J Urol 143 : 1267 ~ 1271
Fromaget C / 1992 / Distribution pattern of con\-nexin 43, a gap junctional protein, during the differentiation of mouse heart myocytes / Differentiation 51 : 9 ~ 20
Haefliger JA / 2002 / Connexins 43 and 26 are differentially increased after rat bladder outlet obstruction / Exp Cell Res 274 : 216 ~ 225
Haferkamp A / 2004 / Increased expression of connexin 43 in the overactive neuro\-genic detrusor / Eur Urol 46 : 799 ~ 805
이 논문의 저자 정보
최근 본 자료
댓글(0)
0