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논문 기본 정보

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학술저널
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한국동물위생학회(구 한국가축위생학회) 한국가축위생학회지 한국가축위생학회지 제35권 제1호
발행연도
2012.1
수록면
9 - 17 (9page)

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The objective of the present study was to isolate and identify infectious bursal disease viruses (IBDVs) from broiler and layer chickens of outbreaks of infectious bursal disease (IBD) in three districts of Bangladesh. A total of 70 bursal samples were collected from dead broiler (n=40) and layer (n=30) chickens showing specific lesions of IBD from seven commercial poultry farms of three different districts (Mymensingh, Chittagong and Tangail) of Bangladesh during the year 2007. Five representative bursal samples from each farm were used for the isolation of IBDVs using 9-day-old embryonated eggs of seronegative flock of layer birds and for identification the samples were subjected to agar gel immunodiffusion test (AGIDT), immunohistochemistry (IHC) and reverse transcription-polymerase chain reaction (RT-PCR). Out of 35 bursal samples, IBDVs were successfully isolated from 28 (80%) samples. By AGIDT, 32 (91.4%) samples were found positive for IBDV antigen. Results of AGIDT clearly indicated that IBDVs detected in 29 bursal samples of six affected farms were identical to each other but not to IBDVs present in the remaining three samples of another farm. Indirect immunoperoxidase staining of the bursal sections revealed the presence of IBDV antigen in 32 (91.4%) samples and the IBDV antigen was detected mainly in the cortex of the lymphoid follicles of the bursal tissues. In histopathology, cell depletion, atrophy and necrosis were observed in many bursal follicles with severe edema of interfollicular septa. Of the 35 bursal samples, 34 (97.1%) samples generated 254 bp product by RT-PCR. In conclusion, the results of virus isolation and identification by AGIDT, IHC and the analysis of viral genome by RT-PCR confirmed the outbreaks of acute IBD in commercial poultry of Bangladesh. Moreover, histopathological findings and results of AGIDT gave a clear indication that the isolates from six outbreaks were different from classical strain and it seems to be of very virulent strain. On the other hand, the isolates from the other outbreak were similar to the classical strain.
#Infectious bursal disease virus #Isolation #Immunohistochemistry #RT-PCR

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참고문헌 (23)

참고문헌 신청
Abdel-Alim GA. / 2001 / Immunogenicity and anti genicity of very virulent strains of infectious bursal disease viruses / Avian Dis 45 : 92 ~ 101 google schola Chettle N. / 1989 / Outbreak of virulent infectious bursal disease in East Anglia / Vet Rec 125 : 271 ~ 272 google schola Cruz-Coy JS. / 1993 / Immunohistochemical detection of infectious bursal disease virus in formalin- fixed, paraffin-embedded chicken tissues using monoclonal antibody / Avian google schola Faragher JT. / 1974 / Immunosuppressive effect of infectious bursal agent in vaccination against Newcastle disease / Vet Rec 95 : 385 ~ 388 google schola Hassan MK. / 1996 / Pathogenicity, attenuation, and immunogenicity of infectious bursal disease virus / Avian Dis 40 : 567 ~ 571 google schola

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