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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 313 - 317 (5page)
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Due to the increase in incidence of infection of Mycobacterium tuberculosis complex (MTC), it is imperative that a rapid diagnosis accompanies the handling of MTC. This is due to the three to eight weeks it takes to culture Mycobacteria, and the lack of sensitivity of microscopic examination of AFB. Recently, nested PCR has been used to detect and diagnose mycobacteria. It is especially useful in complementing diagnosis by histological extra pulmonary. After culturing all the specimens and practicing the nested PCR, we did comparison analysis between nested PCR and culture. There were 76 specimens, 31 of which were positive. Of the 31 positive specimens in culturing, only 22 were positive in nested PCR. Of the 45 negative specimens, 36 were negative in nested PCR. As a result, Sensitivity was 71% and specificity was 80%. Furthermore, the positive predictive value was 71% and negative predictive value was 80%. These results indicate that nested PCR based techniques are sensitive, specific, and rapid methods for the detection of MTC.
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참고문헌 (21)
참고문헌 신청
/ 1995 / Treatment of tuberculosis guidelines for national programmers / World Health Organization
Boddinghaus B / 1990 / Detection and identification of mycobacteria by amplification of rRNA / J Clin Microbiol 28 : 1751 ~ 1759
Brisson-Noel A / 1991 / Diagnosis of tuberculosis by DNA amplification in clinical practice evaluation / Lancet 338 : 364 ~ 366
Brosch R / 2002 / A new evolutionary scenario for the Mycobacterium tuberculosis complex / Proc Natl Acad Sci 99 : 3684 ~ 3689
Chan CM / 1996 / Single-tube nested PCR in the diagnosis of tuberculosis / J Clin Pathol 49 : 290 ~ 294
Boddinghaus B / 1990 / Detection and identification of mycobacteria by amplification of rRNA / J Clin Microbiol 28 : 1751 ~ 1759
Brisson-Noel A / 1991 / Diagnosis of tuberculosis by DNA amplification in clinical practice evaluation / Lancet 338 : 364 ~ 366
Brosch R / 2002 / A new evolutionary scenario for the Mycobacterium tuberculosis complex / Proc Natl Acad Sci 99 : 3684 ~ 3689
Chan CM / 1996 / Single-tube nested PCR in the diagnosis of tuberculosis / J Clin Pathol 49 : 290 ~ 294
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