지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 17 - 23 (7page)
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초록· 키워드
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A fine needle aspiration biopsy (FNAB) is the primary means of distinguishing benign from malignant in thyroid nodules. However, between 10 and 30% of the FNABs of thyroid nodules are diagnosed as 'indeterminate'. A molecular method is needed to reduce unnecessary surgery in this group. In Korea, most thyroid cancer is classic papillary type and BRAFV600E mutation is highly prevalent. Thus, this study compared the pyrosequencing method with the conventional direct DNA sequencing and PCR-RFLP analysis and investigated the evaluation of preoperative BRAFV600E mutation analysis as an adjunct diagnostic method with routine FNABs. Sixty-five (78.3%) of 83 histopathologically diagnosed malignant nodule revealed positive BRAFV600E mutation on pyrosequencing analysis. In detail, 65 (83.8%) of 78 papillary thyroid carcinomas sample showed positive BRAFV600E mutation. None of 29 benign nodules had in pyrodequencing, direct DNA sequencing and PCR-RFLP. Out of 31 thyroid nodules classified as 'indeterminate' on cytological examination preoperatively, 28 cases turned out to be malignant: 24 papillary thyroid carcinomas. Among that, 16 (66.7%) classic papillary thyroid carcinomas had BRAFV600E mutation. Among 65 papillary thyroid carcinomas with positive BRAFV600E mutation detected by pyrosequencing analysis, each 3 cases and 5 cases did not show BRAFV600E mutation by direct DNA sequencing and PCR-RFLP analysis. Therefore, pyrosequencing was superior to direct DNA sequencing and PCR-RFLP in detecting the BRAFV600E mutation of thyroid nodules (p =0.027). Detecting BRAFV600E mutation by pyrosequencing was more sensitivity, faster than direct DNA sequencing or PCR-RFLP.
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참고문헌 (29)
참고문헌 신청
오서영 / 2014 / Improved DNA Extraction Method for Molecular Diagnosis from Smaller numbers of Cells / 대한임상검사과학회지 46(3):99~105
Peyssonnaux C / 2001 / The Raf/MEK/ERK pathway: new concepts of activation / Biol Cell 93:53~62
Xing, M / 2005 / BRAF mutation in thyroid cancer / Endocr Relat Cancer 12:245~262
Son-IlPak / 2002 / Application of Receiver Operating Characteristic(ROC) Curves for Clinical Diagnostic Tests / 한국임상수의학회지 19(3):312~315
/ 2002 / The Ras-Raf-MEK-ERK pathway in the treatment of cancer. / Onkologie 25 : 511 ~ 518
Peyssonnaux C / 2001 / The Raf/MEK/ERK pathway: new concepts of activation / Biol Cell 93:53~62
Xing, M / 2005 / BRAF mutation in thyroid cancer / Endocr Relat Cancer 12:245~262
Son-IlPak / 2002 / Application of Receiver Operating Characteristic(ROC) Curves for Clinical Diagnostic Tests / 한국임상수의학회지 19(3):312~315
/ 2002 / The Ras-Raf-MEK-ERK pathway in the treatment of cancer. / Onkologie 25 : 511 ~ 518
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