지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 151 - 156 (6page)
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초록· 키워드
오류제보하기
Porcine reproductive and respiratory syndrome virus (PRRSV) causes major economic losses to the Korean pig industry. ELISA tests using recombinant nucleocapsid protein of PRRSV have been most commonly used for PRRS diagnostics. In the current study, two commercial PRRSV ELISA kits (Bionote PRRSV Antibody ELISA and IDEXX 3XR PRRS Antibody ELISA) have been compared us-ing sera collected from 19 swine farms in various stages of PRRSV infection confirmed by professional diagnostic centers. Thus 130 sera collected from 5 different farms with active PRRSV infection, 130 sera from 6 different farms with PRRS-stabilized status, and 140 sera from 8 different farms with PRRS-free status were evaluated to determine the correlation of test results between those ELISA kits. Both ELISA kits showed a good correlation [PRRSV-positive farms (R2=0.6375) and stabilized farms (R2=0.8928)] in sample-to-positive (S/P) ratio va lues. Among the 140 sera from negative farms, one sample was falsely positive by either of the ELISA kits. In conclusion, both of the ELISA kits showed a good correlation when applied on field samples collected from farms at various stages of PRRSV infection. Bionote ELISA or IDEXX ELISA gave a false positive result on 1 out of 140 negative sam-ples so their specificity was calculated as 99.3%. Therefore, Bionote ELISA would be a good comple-mentary and alternative method for IDEXX ELISA kit, and vice versa.
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Albina E / 1997 / Epidemiology of porcine reproductive and respiratory syndrome(PRRS) : an overview / Vet Microbiol 55 : 309 ~ 316
Baron T / 1992 / Report on the first outbreaks of the porcine reproductive and respiratory syndrome(PRRS)in France. Diagnosis and viral isolation / Ann Rech Vet 23 : 161 ~ 16
Benfield DA / 1992 / Characterization of swine infertility and respiratory syndrome(SIRS)virus / J Vet Diagn Invest 4 : 127 ~ 133
Chen C / 2013 / Development of a recombinant N-Gp5c fusion protein-based ELISA for detection of antibodies to porcine reproductive and respiratory syndrome virus / J Virol Me
Cho SH / 1993 / Seroprevalence of indirect fluorescent antibody to porcine reproductive and respiratory syndrome virus in selected swine herds / J Vet Diagn Invest 5 : 259 ~
Baron T / 1992 / Report on the first outbreaks of the porcine reproductive and respiratory syndrome(PRRS)in France. Diagnosis and viral isolation / Ann Rech Vet 23 : 161 ~ 16
Benfield DA / 1992 / Characterization of swine infertility and respiratory syndrome(SIRS)virus / J Vet Diagn Invest 4 : 127 ~ 133
Chen C / 2013 / Development of a recombinant N-Gp5c fusion protein-based ELISA for detection of antibodies to porcine reproductive and respiratory syndrome virus / J Virol Me
Cho SH / 1993 / Seroprevalence of indirect fluorescent antibody to porcine reproductive and respiratory syndrome virus in selected swine herds / J Vet Diagn Invest 5 : 259 ~
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