지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
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- 1 - 6 (6page)
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Since the 1980's, several kinds of inactivated bovine viral diarrhea virus (BVDV) vaccines have been used to immunize domestic animals such as cattle and goat in Korea. Immunogenicity of the BVDV vaccines has been checked by the Korean Veterinary Authority using laboratory animals. In this study, we applied a molecular method to investigate the genetic characterization of the BVDV genes in six commercial inactivated BVDV vaccines, and determined the efficiency of two extraction reagents (i.e., sodium citrate or isopropyl myristate) to separate the vaccine antigens from the antigen/adjuvant complexes. Six partial non-coding regions (288 bp) were successfully amplified with specific primer sets, which demonstrated that sodium citrate is more efficient in extracting viral RNA from inactivated gel vaccines than isopropyl myristae. In addition, we identified the virus strains from the vaccines by analyzing the nucleotide sequences of the 5' non-coding region (NCR) of BVDV. The nucleotide similarity of the partial 5' NCR ranged from 95.1 to 100% among BVDV vaccine strains, respectively, indicating that a few manufacturers used different BVDV strains to produce their vaccines.
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참고문헌 (23)
참고문헌 신청
Brock KV / 1991 / Quantitation of bovine viral diarrhea virus in embryo transfer flush fluids collected from a persistently infected heifer / J Vet Diagn Invest 3 : 99 ~ 100
Choi KS / 2011 / Epidemiological observations of bovine viral diarrhea virus in Korean indigenous calves / Virus Gene 42 : 64 ~ 70
Dong-Kun Yang / 2007 / Genetic Typing of Bovine Viral Diarrhea Viruses (BVDV) Circulating in Korea / Journal of Bacteriology and Virology 37 (3) : 138 ~ 147
European pharmacopoeia / 2007 / Strasbourg, European Directorate for the Quality of Medicines & Healthcare. 6th ed : 880 ~ 881
Greiser-Wilke I / 2003 / Bovine viral diarrhoea eradication and control programmes in Europe / Biologicals 31 : 113 ~ 118
Choi KS / 2011 / Epidemiological observations of bovine viral diarrhea virus in Korean indigenous calves / Virus Gene 42 : 64 ~ 70
Dong-Kun Yang / 2007 / Genetic Typing of Bovine Viral Diarrhea Viruses (BVDV) Circulating in Korea / Journal of Bacteriology and Virology 37 (3) : 138 ~ 147
European pharmacopoeia / 2007 / Strasbourg, European Directorate for the Quality of Medicines & Healthcare. 6th ed : 880 ~ 881
Greiser-Wilke I / 2003 / Bovine viral diarrhoea eradication and control programmes in Europe / Biologicals 31 : 113 ~ 118
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