지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 169 - 174 (6page)
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초록· 키워드
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Japanese encephalitis virus (JEV) is one of the main causes of viral encephalitis in human and animals. For over 30 years, a live attenuated JEV vaccine strain has been used in the veterinary field, and it is required to conduct quality evaluation studies on the commercial vaccines. For the quality control of live attenuated JEV vaccine, we investigated the nucleotide sequence similarity of prME gene derived from five JEV vaccines commercially available in pigs in Korea. The Vero cells infected with JEV vaccines showed specific cytopathic effect, which was characterized by rounding and detached cells. In the phylogenetic analysis, all of the vaccine strains showed a close relationship with the original vaccine seed strain (Anyang 300) and clustered into the genotype 3. In comparison of the nucleotide and deduced amino acid sequences of prME genes with the original strain, all JEV vaccine strains showed high amino acid similarity ranging from 98.9% to 99.5%, but had several point mutations, probably due to high mutation rates of viral RNA polymerase by several virus passages. Even though the current JEV vaccine strains have been maintained and produced for a long period of time, the genetic characterization of them have been rarely changed. However, since the mid 1990’s, molecular epidemiology of JEV has been changed sharply from genotype 3 to genotype 1 in Korea, further studies on new vaccine strains to genotype 1 is required for more effective prevention in the field.
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참고문헌 (19)
참고문헌 신청
Baldev R. Gulati / 2012 / Isolation and genetic characterization of Japanese encephalitis virus from equines in India / Journal of Veterinary Science 13 (2) : 111 ~ 118
Beasley DW. / 2008 / Current use and development of vaccines for Japanese encephalitis / Expert Opin Biol Ther 8 : 95 ~ 106
Campbell GL. / 2011 / Estimated global incidence of Japanese encephalitis: a systematic review / Bull World Health Organ 89 : 766 ~ 774
Deng X. / 2011 / Characterization of nonstructural protein 3 of a neurovirulent Japanese encephalitis virus strain isolated from a pig / Virol J 8 : 209 ~
Dong Kun Yang / 2004 / Molecular characterization of full-length genome of Japanese encephalitis virus(KV1899) isolated from pigs in Korea / Journal of Veterinary Science 5
Beasley DW. / 2008 / Current use and development of vaccines for Japanese encephalitis / Expert Opin Biol Ther 8 : 95 ~ 106
Campbell GL. / 2011 / Estimated global incidence of Japanese encephalitis: a systematic review / Bull World Health Organ 89 : 766 ~ 774
Deng X. / 2011 / Characterization of nonstructural protein 3 of a neurovirulent Japanese encephalitis virus strain isolated from a pig / Virol J 8 : 209 ~
Dong Kun Yang / 2004 / Molecular characterization of full-length genome of Japanese encephalitis virus(KV1899) isolated from pigs in Korea / Journal of Veterinary Science 5
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