지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2012.1
- 수록면
- 289 - 294 (6page)
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Mycoplasmas are highly fastidious bacteria, difficult to culture and slow growing. Infections with Mycoplasma species can cause a variety of problems in living organisms and in vitro cell cultures. In this study, we investigated the usefulness of a genus-specific consensus PCR analysis method to detect Mycoplasma species. The developed consensus primer pairs MycoF and MycoR were designed specifically to amplify the 16S ribosomal RNA gene (rRNA) of Mycoplasma species by the optimized PCR system. The developed consensus PCR system effectively amplified 215 bp of Mycoplasma genus-specific region of 16S rRNA. In conclusion, we recommend this consensus PCR for monitoring Mycoplasma species in animals, human and cell culture system.
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참고문헌 (26)
참고문헌 신청
Razin S. / 2010 / Highlights of mycoplasma research-an historical perspective / Biologicals 38:183~190
Taylor-Robinson D. / 1981 / Urogenital mycoplasma infections of man: a review with observations on a recently discovered mycoplasma / Isr J Med Sci 17:524~530
Uphoff CC. / 2011 / Detecting my coplasma contamination in cell cultures by polymerase chain reaction / Methods Mol Biol 731:93~103
Volokhov DV. / 2011 / Mycoplasma testing of cell substrates and biologics: review of alternative non-microbiological techniques / Mol Cell Probes 25:69~77
Armstrong CH. / 1987 / between Mycoplasma hyopneumoniae and Mycoplasma flocculare: practical implications for the serodiagnosis of mycoplasmal pneumonia of swine / Isr J Med
Taylor-Robinson D. / 1981 / Urogenital mycoplasma infections of man: a review with observations on a recently discovered mycoplasma / Isr J Med Sci 17:524~530
Uphoff CC. / 2011 / Detecting my coplasma contamination in cell cultures by polymerase chain reaction / Methods Mol Biol 731:93~103
Volokhov DV. / 2011 / Mycoplasma testing of cell substrates and biologics: review of alternative non-microbiological techniques / Mol Cell Probes 25:69~77
Armstrong CH. / 1987 / between Mycoplasma hyopneumoniae and Mycoplasma flocculare: practical implications for the serodiagnosis of mycoplasmal pneumonia of swine / Isr J Med
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