지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 247 - 252 (6page)
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초록· 키워드
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The economic impact of swine mycoplasma infection is high. An accurate diagnosis is often difficult and time consuming. We report the development and validation of an effective multiplex polymerase chain reaction (PCR) assay that detects Mycoplasma (M.) hyopneumoniae and M. hyorhinis. The multi detection of M. hyopneumoniae and M. hyorhinis primer set were employed to detect mycoplasma spe-cies and typing of the species was performed on the basis of sequence analysis of the PCR product. The target nucleic acid fragments were specifically amplified by M. hyopneumoniae and M. hyorhinis PCR with 16S ribosomal DNA primers. Single and mixed Mycoplasma species DNA templates were used to evaluate the specificity of the multiplex assay. The corresponding specific DNA products were amplified for each pathogen. The multiplex PCR assay provides a novel tool for simultaneous detection and differentiation of M. hyopneumoniae and M. hyorhinis.
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참고문헌 (21)
참고문헌 신청
Barile MF / 1974 / The identification and sources of mycoplasmas isolated from contaminated cell culture / Ann NY Acad Sci 225 : 251 ~ 264
Eldering JA / 2004 / Development of a PCR method for mycoplasma testing of Chinese hamster ovary cell cultures used in the manufacture of recombinant therapeutic proteins / B
Friis NF / 1975 / Some recommendations concerning primary isolation of Mycoplasma suipneumoniae and Mycoplasma flocculare a survey / Nord Vet Med 27 : 337 ~ 339
Friis NF / 1994 / Mycoplasma hyorhinis in the etiology of serositis among piglets / Acta Vet Scand 35 : 93 ~ 98
Harasawa R / 1993 / Detection and tentative identification of dominant Mycoplasma species in cell cultures by restriction analysis of the 16S-23S rRNA intergenic spacer regio
Eldering JA / 2004 / Development of a PCR method for mycoplasma testing of Chinese hamster ovary cell cultures used in the manufacture of recombinant therapeutic proteins / B
Friis NF / 1975 / Some recommendations concerning primary isolation of Mycoplasma suipneumoniae and Mycoplasma flocculare a survey / Nord Vet Med 27 : 337 ~ 339
Friis NF / 1994 / Mycoplasma hyorhinis in the etiology of serositis among piglets / Acta Vet Scand 35 : 93 ~ 98
Harasawa R / 1993 / Detection and tentative identification of dominant Mycoplasma species in cell cultures by restriction analysis of the 16S-23S rRNA intergenic spacer regio
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