지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 333 - 339 (7page)
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초록· 키워드
오류제보하기
Loop-mediated isothermal amplification (LAMP) was developed to detect Mycobacterium tuberculosis complex (MTC) and non-tuberculous mycobacterium (NTM) genomic DNA in blood samples of Korea native cattle. A set of four primers, two outer and two inner, were designed from M. bovis and M. avium genomic DNA targeting the IS6110 and 16S rRNA gene, respectively. Based on 85 Intradermal Tuberculin Test (ITT) positive blood sample and using conventional PCR and LAMP, the agreement quotient (kappa), which measures agreement beyond chance were 0.93 (conventional PCR) and 0.97 (LAMP), respectively. The detection limit of the LAMP method was 2.0×10² copy/ml M. bovis and M. avium cells, compared to 2.0×10³ copy/ml M. bovis and M. avium cells for conventional PCR. These results suggest that the LAMP is a powerful tool for rapid, sensitive, and practical detection of MTC and NTM in blood samples of Korea native cattle.
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등록된 정보가 없습니다.
참고문헌 (13)
참고문헌 신청
Altman DG / 1999 / Practical statistics for medical research / Chapman & Hall/CRC Press
Biet F / 2005 / Zoonotic aspects of Mycobacterium bovis and Mycobacterium avium-intracellulare complex (MAC) / Vet Res 36 : 411 ~ 436
LoBue PA / 2010 / Tuberculosis in humans and animals: an overview / Int J Tuberc Lung Dis 14 : 1075 ~ 1078
Notomi T / 2000 / Loop-mediated isothermal amplification of DNA / Nucleic Acids Res 28 : 63 ~
Schiller I / 2010 / Bovine tuberculosis: a review of current and emerging diagnostic techniques in view of their relevance for disease control and eradication / Transbound E
Biet F / 2005 / Zoonotic aspects of Mycobacterium bovis and Mycobacterium avium-intracellulare complex (MAC) / Vet Res 36 : 411 ~ 436
LoBue PA / 2010 / Tuberculosis in humans and animals: an overview / Int J Tuberc Lung Dis 14 : 1075 ~ 1078
Notomi T / 2000 / Loop-mediated isothermal amplification of DNA / Nucleic Acids Res 28 : 63 ~
Schiller I / 2010 / Bovine tuberculosis: a review of current and emerging diagnostic techniques in view of their relevance for disease control and eradication / Transbound E
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