지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2011.1
- 수록면
- 417 - 428 (12page)
이용수
초록· 키워드
오류제보하기
Species-specific PCR assay was developed for detection of cattle, sheep, goat, horse, dog, pig, chicken, duck, goose, and turkey using mitochondrial 12S rRNA and 16S rRNA as target genes. Also, an internal positive control was used to detect possible false negatives by using 18S rRNA gene. We designed species-specific primers with amplicon length of 190, 219, 350, 467, 241, 119, 171, 229, 111 and 268 bp for cattle, sheep, goat, horse, dog, pig, chicken, duck, goose, and turkey respectively. The specificity of the primers was tested against the other 10 non-target animal species and a cross-reaction was not observed. We developed two multiplex PCR assays for the simultaneous identification of Korea's major livestock species (cattle, pig, chicken and duck) and poultry species (chicken, duck, goose and turkey) from analogous samples, retaining the same specificity. The limit of detection of the multiplex PCR assay (cattle, pig, chicken and duck) ranged between 1 pg and 0.1 pg of template DNA extracts from raw meat. Applying multiplex PCR assays to DNA extracts from experimental pork/beef and pork/chicken tested raw and heat-treated (120℃ for 30 min) mixtures respectively, detection limit was 0.1% level beef in pork, pork in beef and chicken in pork and 1.0% level pork in chicken. In conclusion, this assay using gel-based capillary electrophoresis would be very useful in highly sensitive and rapid identification of animal species or ingredients in minced meat and other meat products.
목차
등록된 정보가 없습니다.
참고문헌 (25)
참고문헌 신청
Ballin NZ / 2010 / Authentication of meat and meat products / Meat Science 86 : 577 ~ 587
Bottero MT / 2011 / Animal species identification in food products: evolution of biomolecular methods / Vet J 190 : 34 ~ 38
Calvo JH / 2001 / Random amplified polymorphic DNA fingerprints for identification of species in poultry pate / Poult Sci 80 : 522 ~ 524
Dalmasso A / 2004 / A multiplex PCR assay for the identification of animal species in feedstuffs / Mol Cell Probes 18 : 81 ~ 87
DiMauro S / 2007 / Mitochondrial DNA Medicine / Biosci Rep 27 : 5 ~ 9
Bottero MT / 2011 / Animal species identification in food products: evolution of biomolecular methods / Vet J 190 : 34 ~ 38
Calvo JH / 2001 / Random amplified polymorphic DNA fingerprints for identification of species in poultry pate / Poult Sci 80 : 522 ~ 524
Dalmasso A / 2004 / A multiplex PCR assay for the identification of animal species in feedstuffs / Mol Cell Probes 18 : 81 ~ 87
DiMauro S / 2007 / Mitochondrial DNA Medicine / Biosci Rep 27 : 5 ~ 9
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