지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.1
- 수록면
- 66 - 72 (7page)
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Fine Needle Aspiration Biopsy Cytology (FNABC), which is known as the most accurate and cost-effective method for diagnosis of the thyroid nodule, may still result in indeterminate cases that are cellular paucity and show minor nuclear atypia. However, most cases are associated with suspicion of papillary thyroid carcinoma (PTC). A B-type Raf kinase (BRAF ) mutation was found in about half of PTCs which is currently helping us to differentiate malignancies from benign lesions. Cases studied included 46 histological, confirmed PTC cases. FNABC 102 cell paucity and 74 atypia benign cases were previously diagnosed as suspicious of PTC using cytologic examination. These cases were analyzed for BRAF mutation by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) with a new restriction enzyme. In this study, the sensitivity and specificity were calculated and, BRAF mutation was detected by means of a histological method in 23 of 46 cases of PTC and no mutation was found in 22 cases. However, one case was not detected. In using FNABC, BRAF mutation was detected in 6 of 102 cases in cell paucity and in 11 of 74 cases in the atypia. Two cases were not detected in the atypia. The sensitivity and specificity of PCR-RFLP in FNABC were 60% and 97.4% respectively. Assessment of Formalin Fixed Paraffin Embedding (FFPE) block demonstrated similarly a 51.1% positive and 48.9% negative in PTC. Evaluation of BRAF mutation revealed high specificity and low sensitivity in using FNABC method. This study suggests that BRAF mutation analysis should be useful for the clinical diagnosis of PTC in FNABC with cytological findings suspicious for PTC.
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참고문헌 (23)
참고문헌 신청
Adeniran AJ / 2006 / Correlation between genetic alterations and microscopic features, clinical manifestations, and prognostic characteristics of thyroid papillary carcinomas
Chung KW / 2006 / Detection of BRAFV600E mutation on fine needle aspiration specimens of thyroid nodule refines cyto-pathology diagnosis, especially in BRAFV600E mutation-pre
Davies L / 2006 / Increasing incidence of thyroid cancer in the United States, 1973-2002 / JAMA 295 : 2164 ~ 2167
Gharib H / 1993 / Fine-needle aspiration biopsy of the thyroid: an appraisal / Ann Intern Med 118 : 282 ~ 289
Jo YS / 2006 / Influence of the BRAF V600E mutation on expression of vascular endothelial growth factor in papillary thyroid cancer / J Clin Endocrinol Metab 91 : 3667 ~ 3670
Chung KW / 2006 / Detection of BRAFV600E mutation on fine needle aspiration specimens of thyroid nodule refines cyto-pathology diagnosis, especially in BRAFV600E mutation-pre
Davies L / 2006 / Increasing incidence of thyroid cancer in the United States, 1973-2002 / JAMA 295 : 2164 ~ 2167
Gharib H / 1993 / Fine-needle aspiration biopsy of the thyroid: an appraisal / Ann Intern Med 118 : 282 ~ 289
Jo YS / 2006 / Influence of the BRAF V600E mutation on expression of vascular endothelial growth factor in papillary thyroid cancer / J Clin Endocrinol Metab 91 : 3667 ~ 3670
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