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논문 기본 정보

자료유형
학술저널
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대한이비인후과학회 Clinical and Experimental Otorhinolaryngology Clinical and Experimental Otorhinolaryngology 제7권 제1호
발행연도
2014.1
수록면
13 - 18 (6page)

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Objectives. Gentamicin (GM) is a commonly used aminoglycoside antibiotic that generates free oxygen radicals within the inner ear, which can cause vestibulo-cochlear toxicity and permanent damage to the sensory hair cells and neurons. Piper longum L. (PL) is a well-known spice and traditional medicine in Asia and Pacific islands, which has been re- ported to exhibit a wide spectrum of activity, including antioxidant activity. In this study, we evaluated the effect of hexane:ethanol (2:8) PL extract (subfraction of PL [SPL] extract) on GM-induced hair cell loss in basal, middle and apical regions in a neonatal cochlea cultures. Methods. The protective effects of SPL extract were measured by phalloidin staining of cultures from postnatal day 2-3 mice with GM-induced hair cell loss. The anti-apoptosis activity of SPL extract was measured using double labeling by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and myosin-7a staining. The radical-scav- enging activity of SPL extract was assessed using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Results. SPL extract at a concentration of 1 µg/mL significantly inhibited GM-induced hair cell loss at basal and middle re- gion of cochlea, while 5 µg/mL was effective against apical region hair cell loss. The protective effect of SPL extract was concentration dependent and hair cells retained their stereocilia in explants treated with SPL extract prior to treat- ment with 0.3 mM GM. SPL extract decreased GM-induced apoptosis of hair cells as assessed by TUNEL staining. The outer hair and inner hair counts were not decreased in SPL extract treated groups in compare to GM treated ex- plants. Additionally, SPL extract showed concentration dependent radical scavenging activity in a DPPH assay. Conclusion. An anti-apoptosis effect and potent radical scavenger activity of SPL extract protects from GM-induced hair cell loss at basal, middle and apical regions in neonatal cochlea cultures.
#Gentamicin #Autotoxicity #Inner hair cell #Outer hair cell #Piper longum #Apoptosis #Antioxidant

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참고문헌 (31)

참고문헌 신청
Shankaracharya NB / 1997 / Characterization of chemical constituents of Indian Long Pepper(Piper-longum L) / J Food Sci Tech 34(1):73~75 google schola Pathak N / 2006 / Modulation of cadmium induced alterations in murine thymocytes by piperine: oxidative stress, apoptosis, phenotyping and blastogenesis / Biochem Pharmacol 72(4):486~497 google schola Pradeep CR / 2003 / Effect of piperine on the inhibition of nitric oxide (NO) and TNF-alpha production / Immunopharmacol Immunotoxicol 25(3):337~346 google schola Jagdale SC / 2009 / Anti-oxidant activity of Piper longum Linn / Int J Biol Chem 3(3):119~125 google schola Joy B / 2010 / Comparison and bioevaluation of Piper longum fruit extracts / J Chem Pharm Res 2(4):612~622 google schola

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