Role of Caffeic Acid on Collagen Production in Nasal Polyp-Derived Fibroblasts

정승원; 박일호; 홍성문; 조정선; 문준혁; 김태훈; 이흥만

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자료유형: 학술저널

저자정보: 정승원 박일호 홍성문 조정선 문준혁 김태훈 이흥만

저널정보: 대한이비인후과학회 Clinical and Experimental Otorhinolaryngology Clinical and Experimental Otorhinolaryngology 제7권 제4호

발행연도: 2014.1

수록면: 295 - 301 (7page)

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Objectives. Caffeic acids are known to have anti-oxidant, anti-inflammatory, immunomodulatory, and tissue reparative ef- fects. The purposes of this study were to determine the effect of caffeic acid on transforming growth factor (TGF) β1- induced myofibroblast differentiation and collagen production, and to determine whether caffeic acid is involved in the antioxidant effect in nasal polyp-derived fibroblasts (NPDFs). Methods. NPDFs were pretreated with caffeic acid (1–10 μM) for 2 hours and stimulated with TGF-β1 (5 ng/mL) for 24 hours. The expression of α-smooth muscle actin (SMA), collagen types I and III, and Nox4 mRNA was determined by a reverse transcription-polymerase chain reaction, and the expression of α-SMA protein was determined by actin ned by immunofluorescence microscopy. The amount of total soluble collagen production was analyzed by the Sircol collagen dye-binding assay. The reactive oxygen species (ROS) generated by NPDFs were determined using 2',7'-di- chlorfluorescein-diacetate. siNox4 was used to determine the effect of Nox4. Results. The expression of α-SMA and production of collagen were significantly increased following TGF-β1 treatment. In contrast, the level of expression of α-SMA and the level of production of collagen were decreased by pretreatment with caffeic acid. The activation of Nox4 and the subsequent production of ROS were also reduced by pretreatment with caffeic acid. The expression of α-SMA was prevented by inhibition of ROS generation with siNox4. Conclusion. Caffeic acid may inhibit TGF-β1-induced differentiation of fibroblasts into myofibroblasts and collagen produc- tion by regulating ROS.

#Nasal polyps #Myofibroblasts #Transforming growth factor beta1 #Caffeic acid #Alpha-smooth muscle actin #Collagen

참고문헌 (18)

참고문헌 신청

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