Dexmedetomidine Preconditioning Attenuates Cisplatin-Induced Ototoxicity in Zebrafish

민두재; 김운영; 하영란; 정인영; 주인영

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자료유형: 학술저널

저자정보: 민두재 김운영 하영란 정인영 주인영

저널정보: 대한이비인후과학회 Clinical and Experimental Otorhinolaryngology Clinical and Experimental Otorhinolaryngology 제7권 제4호

발행연도: 2014.1

수록면: 275 - 280 (6page)

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Objectives. Utilisation of high-frequency drills is known to increase noise induced hearing loss due to increasing the dam- ages of inner ear cells. This study aimed to investigate whether preconditioning by using dexmedetomidine (DEX) decreased the occurrence of ischemia in inner cells of the ear. Methods. We utilised a transgenic zebrafish line Brn3C, and the embryos were collected from breeding adult zebrafish. Five-day-old larvae were cultured at the density of 50 embryos, and the larvae were classified into 4 groups: control, cisplatin group, DEX group, and DEX+yohimbine; adrenoreceptor blocker group. The DEX group was categorised into 3 subgroups by dosage; 0.1, 1, and 10 μM. Preconditioning was performed for 150 minutes and then exposed to cisplatin for 6 hours. The experiment was performed in 7 replicates for each group and the number of hair cells in 3 parts of the neuromasts of each fish was determined. Results. Hair cell apoptosis by cisplatin was attenuated more significantly in the DEX preconditioning group than in the control group. However, the preconditioning effects were not blocked by yohimbine. Conclusion. The results of this study suggest that hearing loss caused by vibration-induced noise could be reduced by using DEX and may occur through other mechanisms rather than adreno-receptors.

#Preconditioning #Dexmedetomidine #Hearing loss

참고문헌 (22)

참고문헌 신청

Cason BA / 1997 / Anesthetic-induced preconditioning: previous administration of isoflurane decreases myocardial infarct size in rabbits / Anesthesiology 87 (5) : 1182 ~ 119

Chang Y /

Choi J / 2013 / Protective effects of apocynin on cisplatin-induced ototoxicity in an auditory cell line and in zebrafish / J Appl Toxicol 33 (2) : 125 ~ 133

Dahmani S / 2008 / Dexmedetomidine increases hippocampal phosphorylated extracellular signal-regulated protein kinase 1 and 2 content by an alpha 2-adrenoceptor-independent

Engelhard K / 2003 / The effect of the alpha 2-agonist dexmedetomidine and the N-methyl-D-aspartate antagonist S(+)-ketamine on the expression of apoptosis-regulating protein