Dexamethasone Induces Apoptosis of Nasal Polyp-Derived Tissue Cultures through JNK and p38 MAPK activation

이태훈; 남정권; 이호민; 김보영; 강명구; 배우용; 허대영; 박성국

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자료유형: 학술저널

저자정보: 이태훈 남정권 이호민 김보영 강명구 배우용 허대영 박성국

저널정보: 대한이비인후과학회 Clinical and Experimental Otorhinolaryngology Clinical and Experimental Otorhinolaryngology 제7권 제2호

발행연도: 2014.1

수록면: 112 - 118 (7page)

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Objectives. Glucocorticoids, such as dexamethasone (DEX), increase apoptosis in a variety of white cells in nasal polyps and apoptosis is an important factor in the resolution of inflammation. However, the mechanism of glucocorticoids induced apoptosis in nasal polyp remains unclear. In this study the authors evaluated which pathways were engaged in apoptosis induced by DEX in an ex vivo model of nasal polyps. Methods. Nasal polyp tissues were cultured using an air-liquid interface method. Cultures were maintained in the absence or presence of DEX (10 or 100 μM) for 24 hours. To investigate the involvement of the apoptotic signaling pathways in nasal polyp, such as caspase cascades, Fas-FasL signaling pathway, mitochondrial pathway and p38 mitogen-acti- vated protein kinase (MAPK)/JNK pathway, the authors performed reverse transcription-polymerase chain reaction and Western blotting. Results. The expression ratios of FasL, activated form of caspase-8, caspase-9, and caspase-3 were significantly higher in DEX-treated polyps (P<0.01). In the Bcl-2 family expression, the anti-apoptotic molecules, Bcl-2 and Bcl-XL de- creased, but pro-apoptotic molecules, Bax increased, and Bid and Bad were activated. In the conventional MAPKs, JNK, and the phospho-p38 MAPK were significantly higher, but phospho-extracellular signal-regulated kinase (ERK)1/2 was significantly lower in DEX-treated polyps (P<0.01). Conclusion. DEX induces apoptosis of nasal polyp via caspase cascades, Fas-FasL signaling pathway, mitochondrial path- way and p38 MAPK/JNK pathway.

#Apoptosis #Glucocorticoids #Nasal polyps #Organ culture #Ex vivo #MAPKs

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참고문헌 신청

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