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Objectives. Cartilage reshaping by laser irradiation is used to correct septal and auricular cartilage deformities. Chondrocyteviability following laser irradiation and reshaping has been well established. However, the regeneration process ofchondrocyte after laser irradiation has not been revealed yet. The aims of this study were to determine the mechanismof cartilaginous thermal injury and the regenerative process of damaged cartilage following laser irradiation. Methods. Laser irradiation was performed on human septal cartilage and rabbit auricular cartilage using a 1,460-nm diodelaser. We observed change in the shape of cartilage and evaluated the extent of cartilage injury using live/dead cell assayvia confocal microscopy. Hoechst and propidium iodide (PI) staining was used to evaluate the mechanism of chondrocyteinjury after laser irradiation. To evaluate the regeneration of cartilage, laser irradiated cartilages were reimplantedinto a subperichondrial pocket and were harvested at 1, 2, and 4 weeks after reimplantation for viability assessmentand histologic examination. Results. Laser irradiation using a 1,460-nm diode laser produced a marked shape change in both human septal and rabbitauricular cartilages. Thermal damage on cartilage was correlated with the exposure time and the laser power. Hoechstand PI staining showed that chondrocyte death by laser irradiation was due to mainly necrosis, rather than apoptosis. In lower power treatment group (0.3 W and 0.5 W), all the chondrocytes regenerated within 4 weeks, however, in 1W treatment group, chondrocytes could not regenerate until 4 weeks. Conclusion. Reshaping of cartilage using 1,460 nm diode laser was attained concurrently with the thermal injury to thechondrocytes. The extent of thermal damage on chondrocytes was dependent on the exposure time and the laser powerand the damaged chondrocytes irradiated with lower level of laser power could be regenerated after reimplantationinto subperichondrial pocket.

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