Detection of MAGE and SSX Gene Expressions by  RT-nested PCR Using Common Primers in Head and  Neck Cancer

송달원; 김동은; 신승진; 정승곤; 이강대

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자료유형: 학술저널

저자정보: 송달원 김동은 신승진 정승곤 이강대

저널정보: 대한이비인후과학회 Clinical and Experimental Otorhinolaryngology Clinical and Experimental Otorhinolaryngology 제1권 제2호

발행연도: 2008.1

수록면: 97 - 102 (6page)

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Objectives. The melanoma antigen gene (MAGE) and synovial sarcoma on X chromosome (SSX) gene families are silent in most normal adult tissues, but are expressed in a variety of malignant lesions. Therefore, detection of MAGE and SSX transcription may be useful for the diagnosis of head and neck cancers. The aim of this study is to detect MAGE and SSX gene transcripts of head and neck cancers using the MAGE 1-6 assay and the SSX 1-9 assay. Methods. The transcripts of MAGE 1-6 and SSX 1-9 genes were detected by the MAGE 1-6 assay and the SSX 1-9 assay respectively, in cancer cell lines, cancer tissue, and induced sputum specimens from head and neck cancer patients. Results. The transcripts of MAGE 1-6 and SSX 1-9 genes were detected in 82.8% and 75.9% of head and neck cancer tissues (N=29) respectively, and 96.6% of cancer tissues expressed at least one of MAGE 1-6 or SSX 1-9 genes. In the induced sputum of head and neck cancer patients (N=18), the transcripts of MAGE 1-6 and SSX 1-9 genes were detected in 72.2% and 77.8%, respectively, and 94.4% of the sputum specimens were positive for either the MAGE 1-6 or the SSX 1-9 assay. Conclusion. These results suggest that the combination of MAGE 1-6 and SSX 1-9 assays may be useful in the diagnosis of head and neck cancer.

#MAGE and SSX gene #Head and neck cancer #RT-PCR

참고문헌 (18)

참고문헌 신청

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Bodey B / 2002 / Cancer-testis antigens: promising targets for antigen directed antineoplastic immunotherapy / Expert Opin Biol Ther 2 (6) : 577 ~ 584

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Clark J / 1994 / Identification of novel genes, SYT and SSX, involved in the t(X;18)(p11.2; q11.2) translocation found in human synovial sarcoma / Nat Genet 7 (4) : 502 ~ 508

Eura M / 1995 / Expression of the MAGE gene family in human head-and-neck squamous-cell carcinomas / Int J Cancer 64 (5) : 304 ~ 308

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