지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한이비인후과학회 Clinical and Experimental Otorhinolaryngology Clinical and Experimental Otorhinolaryngology 제7권 제3호
- 발행연도
- 2014.1
- 수록면
- 175 - 180 (6page)
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Objectives. To investigate the effect of interleukin (IL)-1β on matrix metalloproteinase (MMP)-9 expression in cochlea and regulation of IL-1β-mediated MMP-9 expression by dexamethasone and the molecular and signaling mechanisms in- volved.
Methods. House ear institute-organ of Corti 1 (HEI-OC1) cells were used and exposed to IL-1β with/without dexametha- sone. Glucocorticoid receptor antagonist, RU486, was used to see the role of dexamethasone. PD98059 (an extracel- lular signal-regulated kinases [ERKs] inhibitor), SB203580 (a p38 mitogen-activated protein kinases [MAPK] inhibi- tor), SP600125 (a c-Jun N-terminal kinase [JNK] inhibitor) were also used to see the role of MAPKs signaling pathway(s) in IL-1β-induced MMP-9 expression in HEI-OC1 cells. Reverse transcription-polymerase chain reaction and gelatin zymography were used to measure mRNA expression level of MMP-9 and activity of MMP-9, respectively.
Results. Treatment with IL-1β-induced the expression of MMP-9 in a dose- and time-dependent manner. IL-1β (1 ng/mL)- induced MMP-9 expression was inhibited by dexamethasone. Interestingly, p38 MAPK inhibitor, SB203580, signifi- cantly inhibited IL-1β-induced MMP-9 mRNA and MMP-9 activity. However, inhibition of JNKs and ERKs had no effect on the IL-1β-induced MMP-9 expression.
Conclusion. These results suggest that the pro-inflammatory cytokine IL-1β strongly induces MMP-9 expression via activa- tion of p38 MAPK signaling pathway in HEI-OC1 cells and the induction was inhibited by dexamethasone.
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참고문헌 (19)
참고문헌 신청
Chandrasekhar SS / 2001 / Intratympanic dexamethasone for sudden sensorineural hearing loss: clinical and laboratory evaluation / Otol Neurotol 22 (1) : 18 ~ 23
Cho A / 2000 / Mitogen-activated protein kinases mediate matrix metalloproteinase-9 expression in vascular smooth muscle cells / Arterioscler Thromb Vasc Biol 20 (12) : 2527
Cosgrove D / 1996 / Immunohistochemical localization of basement membrane collagens and associated proteins in the murine cochlea / Hear Res 97 (1-2) : 54 ~ 65
Cosgrove D / 1997 / Expression of the major basement membrane-associated proteins during postnatal development in the murine cochlea / Hear Res 105 (1-2) : 159 ~ 170
Dinh CT / 2008 / Dexamethasone protects organ of corti explants against tumor necrosis factor-alpha-induced loss of auditory hair cells and alters the expression levels of ap
Cho A / 2000 / Mitogen-activated protein kinases mediate matrix metalloproteinase-9 expression in vascular smooth muscle cells / Arterioscler Thromb Vasc Biol 20 (12) : 2527
Cosgrove D / 1996 / Immunohistochemical localization of basement membrane collagens and associated proteins in the murine cochlea / Hear Res 97 (1-2) : 54 ~ 65
Cosgrove D / 1997 / Expression of the major basement membrane-associated proteins during postnatal development in the murine cochlea / Hear Res 105 (1-2) : 159 ~ 170
Dinh CT / 2008 / Dexamethasone protects organ of corti explants against tumor necrosis factor-alpha-induced loss of auditory hair cells and alters the expression levels of ap
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