Hematoxylin and Eosin Staining for Detecting Biofilms: Practical and Cost-Effective Methods for Predicting Worse Outcomes After Endoscopic Sinus Surgery

홍상덕; 동헌종; 정승규; 김효열; 박준오; 하상윤

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자료유형: 학술저널

저자정보: 홍상덕 동헌종 정승규 김효열 박준오 하상윤

저널정보: 대한이비인후과학회 Clinical and Experimental Otorhinolaryngology Clinical and Experimental Otorhinolaryngology 제7권 제3호

발행연도: 2014.1

수록면: 193 - 197 (5page)

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Objectives. Although biofilms have been implicated in poor prognosis after endoscopic sinus surgery (ESS), traditional methods detecting biofilm such as scanning electron microscope and confocal scanning laser microscope were rarely used in the practice. The aims of this study was to determine whether the presence or absence of a biofilm detected by hematoxylin and eosin (H&E) staining followed by light microscopy (LM) that is widely used in daily practice, predicts surgical outcomes after ESS. Methods. Retrospective analysis of prospectively collected data. Fifty-five consecutive adult patients (>18 years) who un- derwent ESS for chronic rhinosinusitis with a minimum of 12-months of follow-up were enrolled in this study. Ran- dom sinonasal mucosal samples were assessed for biofilm presence using H&E staining with LM. Three independent observers scored whether a biofilm was present or absent based on H&E staining/LM, and the interrater variability was calculated. Pre- and postoperative sinus symptoms and sinonasal mucosal grading were assessed. Results. Biofilms were present in 28 patients (51%), and the intraclass correlation coefficient according to H&E staining/ LM was 0.731. The presence of a biofilm was associated with a higher preoperative Lund-MacKay computed tomog- raphy score (22.3 for biofilm-positive patients vs. 18.6 for biofilm-negative patients; P=0.021) and persistent inflam- mation (mucosal edema and discharge) after ESS (P<0.05). Conclusions . The presence or absence of a biofilm based on H&E staining/LM is correlated with disease severity and surgi- cal outcomes after ESS. H&E staining/LM for detecting biofilm could be practical and cost-effective methods for pre- dicting prognosis of ESS.

#Biofilms #Chronic rhinosinusitis #Endoscopic sinus surgery #Hematoxylin-eosin staining #Light microscope #Nasendoscopy #Postsurgi-cal outcomes

참고문헌 (14)

참고문헌 신청

Bendouah Z / 2006 / Biofilm formation by Staphylococcus aureus and Pseudomonas aeruginosa is associated with an unfavorable evolution after surgery for chronic sinusitis and

Chen HH / 2012 / Bacterial biofilms in chronic rhinosinusitis and their relationship with inflammation severity / Auris Nasus Larynx 39 (2) : 169 ~ 174

Foreman A / 2009 / Characterization of bacterial and fungal biofilms in chronic rhinosinusitis / Am J Rhinol Allergy 23 (6) : 556 ~ 561

Foreman A / 2010 / Targeted imaging modality selection for bacterial biofilms in chronic rhinosinusitis / Laryngoscope 120 (2) : 427 ~ 431

Hochstim CJ / 2010 / Biofilm and persistent inflammation in endoscopic sinus surgery / Otolaryngol Head Neck Surg 143 (5) : 697 ~ 698

이 논문의 저자 정보

홍상덕

소속기관 Samsung Medical Center Sungkyunkwan University School of Medicine Seoul Korea

주요연구분야 의약학 > 의학 일반 의약학 > 임상의학 > 이비인후과학

논문수 39 이용수 63

동헌종

소속기관 성균관대학교