지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한이비인후과학회 Clinical and Experimental Otorhinolaryngology Clinical and Experimental Otorhinolaryngology 제6권 제3호
- 발행연도
- 2013.1
- 수록면
- 140 - 145 (6page)
이용수
초록· 키워드
오류제보하기
Objectives. Until recently, most patch-clamp recordings in inner hair cells (IHCs) have been performed at room temperature.
The results acquired at room temperature should be corrected if they are to be related to in vivo findings. However,the temperature dependency to ion channels in IHCs is unknown. The aim of this study was to investigate the effect of temperature on the potassium currents in IHCs.
Methods. IHCs were isolated from a mature guinea-pig cochlea and potassium currents were recorded at room temperature (around 25°C) and physiological temperatures (35°C-37°C).
Results. IHCs showed outwardly rectifying currents in response to depolarizing voltage pulses, with only a slight inward current when hyperpolarized. The amplitude of both outward and inward currents demonstrated no temperature dependency,however, activation and inactivation rates were faster at 36ºC than at room temperature. Half-time for activation was shorter at 36°C than at room temperature at membrane potentials of -10, +10, +20, +30, and +40 mV.
Q10 for the activation rate was 1.83. The inactivation time constant in outward tetraethylammonium-sensitive potassium currents was much smaller at 36°C than at room temperature between the membrane potentials of -20 and +60mV. Q10 for the inactivation time constant was 3.19.
Conclusion. The results of this study suggest that the amplitude of potassium currents in IHCs showed no temperature dependence either in outward or inward-going currents, however, activation and inactivation accelerated at physiological temperatures.
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Armstrong CE / 2001 / Rapidly inactivating and non-inactivating calcium-activated potassium currents in frog saccular hair cells / J Physiol 536 (Pt 1) : 49 ~ 65
Beam KG / 1983 / A quantitative study of potassium channel kinetics in rat skeletal muscle from 1 to 37 degrees C / J Gen Physiol 81 (4) : 485 ~ 512
Brandt A / 2005 / Few CaV1.3 channels regulate the exocytosis of a synaptic vesicle at the hair cell ribbon synapse / J Neurosci 25 (50) : 11577 ~ 11585
Bregestovski P / 1986 / Elevation of intracellular calcium reduces voltage-dependent potassium conductance in human T cells / Nature 319 (6056) : 776 ~ 778
Choquet D / 1987 / Cyclic AMPmodulated potassium channels in murine B cells and their precursors / Science 235 (4793) : 1211 ~ 1214
Beam KG / 1983 / A quantitative study of potassium channel kinetics in rat skeletal muscle from 1 to 37 degrees C / J Gen Physiol 81 (4) : 485 ~ 512
Brandt A / 2005 / Few CaV1.3 channels regulate the exocytosis of a synaptic vesicle at the hair cell ribbon synapse / J Neurosci 25 (50) : 11577 ~ 11585
Bregestovski P / 1986 / Elevation of intracellular calcium reduces voltage-dependent potassium conductance in human T cells / Nature 319 (6056) : 776 ~ 778
Choquet D / 1987 / Cyclic AMPmodulated potassium channels in murine B cells and their precursors / Science 235 (4793) : 1211 ~ 1214
이 논문의 저자 정보
최근 본 자료
댓글(0)
0