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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 대한이비인후과학회 Clinical and Experimental Otorhinolaryngology Clinical and Experimental Otorhinolaryngology 제6권 제3호
- 발행연도
- 2013.1
- 수록면
- 176 - 183 (8page)
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초록· 키워드
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Objectives. Based on studies of the extensive tropism of neural stem cells (NSCs) toward malignant brain tumor, we hypothesized that NSCs could also target head and neck squamous cell carcinoma (HNSCC) and could be used as a cellular therapeutic delivery system.
Methods. To apply this strategy to the treatment of HNSCC, we used a human NSC line expressing cytosine deaminase (HB1.F3-CD), an enzyme that converts 5-fluorocytosine (5-FC) into 5-fluorouracil (5-FU), an anticancer agent. HB1.
F3-CD in combination with 5-FC were cocultured with the HNSCC (SNU-1041) to examine the cytotoxicity on target tumor cells in vitro. For in vivo studies, an HNSCC mouse model was created by subcutaneous implantation of human HNSCC cells into athymic nude mice. HB1.F3-CD cells were injected into mice using tumoral, peritumoral,or intravenous injections, followed by systemic 5-FC administration.
Results. In vitro, the HB1.F3-CD cells significantly inhibited the growth of an HNSCC cell line in the presence of the 5-FC.
Independent of the method of injection, the HB1.F3-CD cells migrated to the HNSCC tumor, causing a significant reduction in tumor volume. In comparison to 5-FU administration, HB1.F3-CD cell injection followed by 5-FC administration reduced systemic toxicity, but achieved the same level of therapeutic efficacy.
Conclusion. Transplantation of human NSCs that express the suicide enzyme cytosine deaminase combined with systemic administration of the prodrug 5-FC may be an effective regimen for the treatment of HNSCC.
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참고문헌 (23)
참고문헌 신청
Aboody KS / 200
Aboody KS / 2000 / Neural stem cells display extensive tropism for pathology in adult brain: evidence from intracranial gliomas / Proc Natl Acad Sci U S A 97 (23) : 12846 ~ 1
Aboody KS / 2006 / Targeting of melanoma brain metastases using engineered neural stem/progenitor cells / Neuro Oncol 8 (2) : 119 ~ 126
Arbab AS / 2003 / Characterization of biophysical and metabolic properties of cells labeled with superparamagnetic iron oxide nanoparticles and transfection agent for cellul
Bulte JW / 2004 / Preparation of magnetically labeled cells for cell tracking by magnetic resonance imaging / Methods Enzymol 386 : 275 ~ 299
Aboody KS / 2000 / Neural stem cells display extensive tropism for pathology in adult brain: evidence from intracranial gliomas / Proc Natl Acad Sci U S A 97 (23) : 12846 ~ 1
Aboody KS / 2006 / Targeting of melanoma brain metastases using engineered neural stem/progenitor cells / Neuro Oncol 8 (2) : 119 ~ 126
Arbab AS / 2003 / Characterization of biophysical and metabolic properties of cells labeled with superparamagnetic iron oxide nanoparticles and transfection agent for cellul
Bulte JW / 2004 / Preparation of magnetically labeled cells for cell tracking by magnetic resonance imaging / Methods Enzymol 386 : 275 ~ 299
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