기니픽 제1형 전정유모세포에서 젠타마이신에 의해 유발되는 전정독성에 대한Epigallocatechin-3-Gallate의 보호 효과

최영호; 이성수; 문석진; 안병현; 김남국; 정한성; 박종성; 조형호; 조용범

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자료유형: 학술저널

저자정보: 최영호 이성수 문석진 안병현 김남국 정한성 박종성 조형호 조용범

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제57권 제4호

발행연도: 2014.1

수록면: 226 - 232 (7page)

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Background and Objectives Gentamicin (GM) is well known for its vestibulotoxicity. There have been many reports about vestibulotoxicity, however, its mechanism is still unclear. So far, it is known that GM affects the voltage-dependent K+ current and nitric oxide (NO) production. Epigallocatechin-3-gallate (EGCG) is the major component of green tea and is knownto have anti-oxidative and anti-toxic effect. This study was undertaken to investigate the protective effect of EGCG against gentamicin on vestibular hair cell (VHC). Materials and Method White guinea pigs (200-250 g) were rapidly decapitated and thetemporal bones were immediately removed. Under a dissecting microscope, the crista ampullaris was obtained. The dissociated VHCs were transferred into a recording chamber mountedonto an inverted microscope. Whole-cell membrane currents and potentials were recorded using standard patch-clamp techniques. In addition, measurements of NO production were obtained using the NO-sensitive dye, 4,5-diamino-fluorescein diacetate (DAF-2DA). Results Type I VHCs Voltage-dependent K+ current was activated from low depolarizingstimulation. As the stimulation increased, higher current was detected. Voltage-dependent K+current in type I VHCs was decreased when GM (200 μM) was administrated and GM effectsof K+ current inhibition was significantly blocked by EGCG. Extracellular GM induced an increase in DAF-2DA fluorescence, which thus indicates NO production in VHCs. Also, the GMinduced NO production was inhibited by EGCG. Conclusion GM inhibits voltage-dependent K+ current by releasing NO in isolated type IVHCs. EGCG blocks this inhibitory effects, suggesting a protective role on GM vestibulotoxicity. Korean J Otorhinolaryngol-Head Neck Surg 2014;57(4):226-32

#Epigallocatechin-3-gallate #Gentamicin #Vestibular hair cell #Vestibular toxicity

참고문헌 (23)

참고문헌 신청

Basile AS / 1996 / N-methyl-D-aspartate antagonists limit aminoglycoside antibiotic-induced hearing loss / Nat Med 2 (12) : 1338 ~ 1343

Basile AS / 1999 / Dizocilpine attenuates streptomycin-induced vestibulotoxicity in rats / Neurosci Lett 265 (2) : 71 ~ 74

Braicu C / 2013 / Epigallocatechin-3-Gallate (EGCG) inhibits cell proliferation and migratory behaviour of triple negative breast cancer cells / J Nanosci Nanotechnol 13 (1)

Chambers HF / 1996 / Goodman & Gilman’s the Pharmacological Basis of Therapeutics / McGraw-Hill, Health Professions Division : 1103 ~ 1121

Chen JW / 2000 / Major potassium conductance in type I hair cells from rat semicircular canals: characterization and modulation by nitric oxide / J Neurophysiol 84 (1) : 139