사람중이점막세포에서 Uridine-5'-Triphosphate 자극에 의한 Cystic Fibrosis Transmembrane  Conductance Regulator와 Ca2+-Activated Chloride Channel을 통한 Chloride 분비 활성화

손은진; 지헌영; 이민구; 이원상; 최재영

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자료유형: 학술저널

저자정보: 손은진 지헌영 이민구 이원상 최재영

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제54권 제12호

발행연도: 2011.1

수록면: 840 - 846 (7page)

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Background and Objectives Nucleotide binding to purinergic P2Y receptors contributes to the regulation of fluid and ion transport in the middle ear epithelial cells. Here, we investigated the regulatory mechanism of the P2Y2 receptor agonist, uridine-5’-triphosphate (UTP), on Cl- transport in cultured normal human middle ear epithelial (NHMEE) cells. Materials and Method Electrophysiological measurements were performed in monolayers of cultured NHMEE cells. Short circuit currents (Isc) were measured from the cells mounted in Ussing chambers under various conditions. Results Apical addition of UTP in presence of amiloride evoked a transient rise and a sustained response in Isc due to Cl- efflux. Application of different Cl- channel blockers to the apical side of the cells significantly decreased UTP-induced Isc. Niflumic acid (NFA), a known blocker of Ca2+-activated chloride channels (CACC), and CFTRinh172, a selective inhibitor of cystic fibrosis transmembrane conductance regulator (CFTR), partially inhibited the UTP-induced Cl- secretion, respectively. Conclusion Cl- transport across the airway epithelia plays a predominant role in regulating airway hydration. In this study, UTP is shown to increase both CACC and CFTR-dependent Cl- secretion in NHMEE cells, suggesting their role in fluid and ion transport in the middle ear epithelium.

#UTP ㆍChloride ㆍIon channel ㆍMiddle ear.

참고문헌 (23)

참고문헌 신청

Boucher RC / 1999 / Molecular insights into the physiology of the ‘thin film’ of airway surface liquid / J Physiol 516 (Pt 3) : 631 ~ 638

Bucheimer RE / 2004 / Purinergic regulation of epithelial transport / J Physiol 555 (Pt 2) : 311 ~ 321

Choi JY / 2006 / ENaCand CFTR-dependent ion and fluid transport in human middle ear epithelial cells / Hear Res 211 (1) : 26 ~ 32

Choi JY / 2007 / Interleukin- 1beta suppresses epithelial sodium channel beta-subunit expression and ENaC-dependent fluid absorption in human middle ear epithelial cells / Eu

Homolya L / 2000 / Cell to cell communication in response to mechanical stress via bilateral release of ATP and UTP in polarized epithelia / J Cell Biol 150 (6) : 1349 ~ 136