재발성 편도염 환자에서 레이저초점 주사현미경과 FISH를 이용한 편도 균막의 분석

김동영; 김선태; 강일규; 정주현; 박동욱; 우주현

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자료유형: 학술저널

저자정보: 김동영 김선태 강일규 정주현 박동욱 우주현

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제54권 제11호

발행연도: 2011.1

수록면: 778 - 783 (6page)

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연구주제

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연구배경

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연구방법

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Background and Objectives Biofilm is an encased colony of bacteria and the bacteria with-in the biofilm have resistance to antibiotics and the humoral immune system. Biofilm is currently thought to play an important role and to be very refractory to conventional therapeutics. The aim of this study is to analyze the relationship between the biofilm of the tonsil and recurrent tonsillitis with a case-control study. Subjects and Method This is a case-control study. The case group included 31 patients who had histories of tonsillitis and the control group included 29 volunteers who had no history of tonsillitis. Confocal laser scanning microscopy was performed to identify biofilms. We compared the prevalence rates of biofilm between the 2 groups. Fluorescent in situ hybridization (FISH) was performed to identify bacteria, using probes for S. aureus, S. pyogenes, H. influenzae, S. pneumoniae, and K. pneumonia. Results In case group, biofilms were found in 22 (71.0%) of 31 and in control group, biofilms were found in 12 (41.4%) of 29. The prevalence rates of biofilm were significantly higher in case group (p=0.036). Twenty nine cases were enrolled in FISH and 12 cases of them were positive for FISH. S. aureus was most commonly identified. Conclusion This study suggests that the biofilms could play a causative role in recurrent tonsillitis, further studies are needed to confirm pathogenic relation between biofilm and recurrent tonsillitis.

#Recurrent ㆍTonsillitis ㆍBiofilm.

참고문헌 (24)

참고문헌 신청

Al-Marzrou KA / 2008 / Adherent biofilms in adenotonsillar diseases in children / Arch Otolaryngol Head Neck Surg 134 (1) : 20 ~ 23

Balogh K / 2007 / Mouth, nose, and paranasal sinuses. In Histology for pathologists. 3rd ed / Lippincott Williams & Wilkins : 403 ~ 430

Chole RA / 2003 / Anatomical evidence of microbial biofilms in tonsillar tissues:a possible mechanism to explain chronicity / Arch Otolaryngol Head Neck Surg 129 (6) : 634 ~

Cochrane DM / 1988 / Antibody response to Pseudomonas aeruginosa surface protein antigens in a rat model of chronic lung infection / J Med Microbiol 27 (4) : 255 ~ 261

Costerton JW / 1995 / Microbial biofilms / Annu Rev Microbiol 49 : 711 ~ 745