소아의 재발성 편도염 조직에서 사이토카인과cyclooxygenase 발현 조절에 관한 연구

홍순관; 김문정; 정성민; 홍영숙; 변성완; 고영민; 김현경

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자료유형: 학술저널

저자정보: 홍순관 김문정 정성민 홍영숙 변성완 고영민 김현경

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제47권 제8호

발행연도: 2004.1

수록면: 765 - 772 (8page)

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Background and Objectives：Prostanoids are converted from arachidonic acid via cyclooxygenase (COX), which has two isoforms, COX-1 and COX-2. It has recently been shown that prostanoids may have a novel role in mediating cytokine release in some cells. We investigated the baseline expresion and regulation of interleukin (IL)-6, IL-8, COX-1 and COX-2 in palatine tonsils from pediatric recurrent tonsillitis. Materials and Method：rent tonsillitis during elective tonsillectomy and adenoidectomy. Tonsil cells were incubated with culture media only or with pro-inflammatory cytokines (IL-1β and TNF-α) or inhibitors of transcription (actinomycin D) or translation (cycloheximide). In these groups, IL-6, IL-8, COX-1, COX-2 mRNAs and COX-1, COX-2 proteins were analyzed by reverse transcriptase-polymerase chain reaction and Western blot, respectively. Results：mRNAs were detected although tonsillectomy was done in the silent phase without acute infection. The culture of tonsil cells with pro-inflamatory cytokines increased the expression of IL-6, IL-8 and COX-2 mRNAs and COX-2 protein. The expres-sions of IL-6, IL-8 and COX-2 mRNAs and COX-2 protein induced by pro-inflammatory cytokines was significantly inhibited by actinomycin D, not by cycloheximide. Conclusion：Tonsil cells isolated from pediatric recurrent tonsillitis are in constantly 6 and IL-8 mRNAs. The regulation of these efects occurs at the transcriptional level. Furthermore, the regulation of cytokines and COX-2 may provide an understanding of mechanism of prostanoids synthesis in chronic tonsillitis.

#사이토카인·편도염. #Cytokines· Prostaglandin-endoperoxide synthase· Tonsillitis.

참고문헌 (20)

참고문헌 신청

/ 1985 / Synthesis of prostaglandin E2and F2α in human tonsillar lymphocytes 47 : 26 ~ 31

/ 1990 / Analysis of cytokinemRNA and DNA:Detection and quantitation by competitive polymerasechain reaction 87 : 2725 ~ 2729

/ 1993 / Expression of a mitogen inducible cyclooxygenase in brain neurons:Regulation by synaptic activity and glucocorticoids 11 : 371 ~ 386

/ 1993 / High frequency of spontaneous interferon-gamma-producingcells in human tonsils:Role of local accessory cells and solublefactors 91 : 157 ~ 163

/ 1994 / Concomitant in vivo production of 19 different cytokines in humantonsils 83 : 16 ~ 24

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