개의 지방조직내 중배엽줄기세포를 이용한 연골세포의 분화

이병주; 서철주; 이진춘; 왕수건; 정진섭; 이량화

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자료유형: 학술저널

저자정보: 이병주 서철주 이진춘 왕수건 정진섭 이량화

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제47권 제8호

발행연도: 2004.1

수록면: 760 - 764 (5page)

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📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

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Background and Objectives：Tisue enginering is presently being utilized in parts of cartilage repair. Sources of cells for tissue enginering are chondrocytes from mature cartilage and bone marrow mesenchymal stem cells that are able to diferentiate into chondrocytes. Recent studies have shown that adipose tisue have mesenchymal stem cels which can diferentiate into adipogenic, chondrogenic myogenic, osteogenic cels and neural cels in vitro. In this study, we have examined chondrogenic potential of the canine adipose tisue-derived mesenchymal stem cell (ATSC). Materials and Method：We harvested canine adipose tisue from the inguinal area. ATSCs were enzymatically released from canine adipose tissue. Under apropriate culture conditions, ATSCs were induced to diferentiate into chondrocyte lineages using micromass culture technique. We used imunostain to type I colagen and toluidine blue stain to confirm chondrogenic diferentiation of ATSCs. Results：We could isolate ATSCs from canine adipose tisue. ATSCs that has positive response to immunostain of type I collagen and toluidine blue stain. Conclusion：In vitro, ATSCs diferentiated into cels that have characteristic cartilage matrix molecules in the presence of lineage-specific induction factors. Adipose tissue may represent an alternative source to bone marow-derived MSCs.

#연골세포·지방조직·조직공학. #Chondrocyte· Adipose tissue· Tisue engineering.

참고문헌 (15)

참고문헌 신청

Cao YL / 1999 / Characteristics of cartilage engineered from human pediatric auricularcartilage 103 : 1111 ~ 1119

Caplan AI / 1991 / Mesenchymal stem cells 9 : 641 ~ 650

Gimble JM / 2002 / Chondrogenic potential of adipose tissue-derived stromal cells in vitroand in vivo 290 : 763 ~ 769

Gomez RB / 1998 / Cartilaginous behavior in nasal surgery:Comparative observationalstudy 40 : 34 ~ 38

Ha EH / 2001 / Tissueengineered cartilage formation using human hyaline chondrocytes andelastic chondrocytes 28 : 233 ~ 240

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