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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 387 - 393 (7page)
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초록· 키워드
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Biotyping of Vibrio vulnificus strains isolated from marine environments along the south coast of Korea showed that the majority of the isolates (94.7%) belonged to biotype 1 and the remaining isolates (5.3%) belonged to biotype 2. Analysis of 16S rRNA V. vulnificus strains isolated from marine environments using a multiplex polymerase chain reaction (PCR) revealed that 78.7% were type A and 21.3% were type B. Random amplified polymorphic DNA (RAPD) was used to analyze the genomic differences in V. vulnificus among the biotype 2 strains isolated from marine environments (newly isolated strains group) and reference strains obtained from infected eels (reference strains group). The two groups had distinctly different profiles of the amplicons produced from RAPD. Additionally, biochemical comparison of these strains revealed that all four strains isolated from marine environments differed from the strains isolated from eels in their ability to promote D-mannitol fermentation. Two (NH 1 and NH 2) out of four isolates of biotype 2 from marine environments showed pathogenicity in eels Anguilla japonica in a challenge test. These isolates did not agglutinate with antisera against V. vulnificus NCIMB 2137 (serovar E), ATCC 27562 (non-serovar E), and ATCC 33816 (atypical serovar E).
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참고문헌 (23)
참고문헌 신청
Amaro C / 1996 / Vibrio vulnificus biotype 2, pathogenic for eels, is an opportunistic pathogen for humans / Appl Environ Microbiol 62 : 1454 ~ 1457
Amaro C / 1999 / Isolation of Vibrio vulnificus biotype 2 from aquatic habitats in Taiwan / Appl Environ Microbiol 65 : 1352 ~ 1355
Aznar R / 1994 / Sequence determination of rRNA genes of pathogenic vibrio species and whole-cell identification of Vibrio vulnificus with rRNA-targeted oligonucleotide probe
Biosca EG / 1996 / Effect of low temperature on starvation-survival of the eel pathogen Vibrio vulnificus biotype 2 / Appl Environ Microbiol 65 : 1117 ~ 1126
Bisharat N / 1999 / Clinical, epidemiological, and microbiological features of Vibrio vulnificus biogroup 3 causing outbreaks of wound infection and bacteraemia in Israel / T
Amaro C / 1999 / Isolation of Vibrio vulnificus biotype 2 from aquatic habitats in Taiwan / Appl Environ Microbiol 65 : 1352 ~ 1355
Aznar R / 1994 / Sequence determination of rRNA genes of pathogenic vibrio species and whole-cell identification of Vibrio vulnificus with rRNA-targeted oligonucleotide probe
Biosca EG / 1996 / Effect of low temperature on starvation-survival of the eel pathogen Vibrio vulnificus biotype 2 / Appl Environ Microbiol 65 : 1117 ~ 1126
Bisharat N / 1999 / Clinical, epidemiological, and microbiological features of Vibrio vulnificus biogroup 3 causing outbreaks of wound infection and bacteraemia in Israel / T
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