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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 1 - 7 (7page)
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초록· 키워드
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Antifreeze proteins (AFPs) lower the freezing point but not the melting point of aqueous solutions by inhibiting the growth of ice crystals via an adsorption-inhibition mechanism. However, the function of type IV AFP (AFP IV) is questionable, as its antifreeze activity is on the verge of detectable limits, its physiological concentration in adult fish blood is too low to function as a biological antifreeze, and its homologues are present even in fish from tropic oceans as well as freshwater. Therefore, we speculated that AFP IV may have gained antifreeze activity not by selective pressure but by chance. To test this hypothesis, we cloned, expressed, and assayed AFP IV from cultured subtropical olive flounder (Paralichthys olivaceus), which do not require antifreeze protein for survival. Among the identified expressed sequence tags of the flounder liver sample, a 5′-deleted complementary DNA (cDNA) sequence similar to the afp4 gene of the longhorn sculpin was identified, and its full-length cDNA and genome structure were examined. The deduced amino acid sequence of flounder AFP IV shared 55, 53, 52, and 49 % identity with those of Pleuragramma antarcticum, Myoxocephalus octodecemspinosus, Myoxocephalus scorpius, and Notothenia coriiceps, respectively. Furthermore, the genomic structure of this gene was conserved with those of other known AFP IVs. Notably, the recombinant AFP IV showed a weak but distinct thermal hysteresis of 0.07 ± 0.01 °C at the concentration of 0.5 mg/mL, and ice crystals in an AFP IV solution grew star-shaped, which are very similar to those obtained from other polar AFP IVs. Taken together, our results do not support the hypothesis of evolution of AFP IV by selective pressure, suggesting that the antifreeze activity of AFP IV may have been gained by chance.
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참고문헌 (29)
참고문헌 신청
Bendtsen JD / 2004 / Improved prediction of signal peptides: SignalP 3.0 / J Mol Biol 340:783~795
Breton TS / 2012 / Identification of ovarian gene expression patterns during vitellogenesis in Atlantic cod(Gadus morhua) / Gen Comp Endocrinol 179:296~304
Cheng CH / 1998 / Evolution of the diverse antifreeze proteins / Curr Opin Genet Dev 8:715~720
Cheng CC / 2003 / Encyclopedia of life support systems (EOLSS)—theme 6.73 extremophiles, developed under the auspices of the UNESCO / Eolss Publishers
Cheng CH / 1999 / Evolution of an antifreeze glycoprotein / Nature 401:443~444
Breton TS / 2012 / Identification of ovarian gene expression patterns during vitellogenesis in Atlantic cod(Gadus morhua) / Gen Comp Endocrinol 179:296~304
Cheng CH / 1998 / Evolution of the diverse antifreeze proteins / Curr Opin Genet Dev 8:715~720
Cheng CC / 2003 / Encyclopedia of life support systems (EOLSS)—theme 6.73 extremophiles, developed under the auspices of the UNESCO / Eolss Publishers
Cheng CH / 1999 / Evolution of an antifreeze glycoprotein / Nature 401:443~444
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